To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
The endospore stain is a process used in bacteriology. It is used to find a bacterial samples endospores so that the bacteria can be classified.
malachite green dye. The dye stains the endospore in the bacteria.
You don't use simple staining techniques for seeing endospores because clear areas could show different things like organelles. Simple staining will only stain the endospore but not inside.
malachite green stain
vital dye in microbiology
The malachite green penetrates the endospores, the counterstain of safranin will stain the vegetative cells. Endospores - greenish-blue Vegetative Cells - Red/Pink
it is a basic dye that will stain the cells. That makes it a positive stain.
it is used to dye materials like silk,leather and paper.And also to:As mineral specimens, an important ore of copper, as an ornamental stone, a pigment and for jewelry.Natural malachite mineral Specimens
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
No, endospores are not acid fast because they are not cells. Endospores will stain with the acid fast stain and look fuchsia but they are not positive for acid fast.
vital dye in microbiology
Green
Pink Dye
The malachite green penetrates the endospores, the counterstain of safranin will stain the vegetative cells. Endospores - greenish-blue Vegetative Cells - Red/Pink
Malachite green
it depends on the animal
Methylene blue
Usually, it does stain.
An organic stain is considered a biological stain using organic dye. Organic dyes used are often modified from the textile industry.
methane blue
Endospores are impermeable to most stains so heat is usually applied to drive the stain into the endospore.