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Why do you use on or slide to be examined with the oil immersion objective?
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
What does the high lens of a microscope only use?
That is the oil lens. For suspension of microbes in an oil solution in which the objective lens is also immersed. Improves resolution.
What happens if you try to use the coarse adjustment when the 10x lens is in place?
One thing that can happen if you use the coarse adjustment while the oil immersion objective is in place is that the slide will end up breaking. It is better to use the fine adjustment.
What would you observe if you forgot to use oil with the oil immersion lens?
Immersion oil is only required when using a light manuscript at 1000 times magnification (10x from occular lens and 100x from objective lens). At 1000x magnification the image will have poor resolution (loook fuzzy) without the use of immersion oil. This is because the cover slip on the sample and air have different refractory indexes. The light scatter that occurs during the transition from glass to air is noticable at such a high magnification. Immersion oil has a refractive index very similar to the cover slip, thus reducing the light scatter as the light passes from the sample to the objective lens.
Why is it necessary to use emmersion oil with the 100x objective?
because at this magnification the light diffraction in air is to important to have enough light reach the lens, so oil should be in the contact between the slide and the lens because it has a much lower refraction index than air allowing more light to reach the lens
Why do you use on or slide to be examined with the oil immersion objective?
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
Which objective focuses closest to the slide when it is in focus?
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
What does the high lens of a microscope only use?
That is the oil lens. For suspension of microbes in an oil solution in which the objective lens is also immersed. Improves resolution.
What happens if you try to use the coarse adjustment when the 10x lens is in place?
One thing that can happen if you use the coarse adjustment while the oil immersion objective is in place is that the slide will end up breaking. It is better to use the fine adjustment.
Why is it important to use the fine adjustment knob when using the highest power objective lens?
So you do not crack the high power objective lens - this lens is very fragile and expensive.
What would you observe if you forgot to use oil with the oil immersion lens?
Immersion oil is only required when using a light manuscript at 1000 times magnification (10x from occular lens and 100x from objective lens). At 1000x magnification the image will have poor resolution (loook fuzzy) without the use of immersion oil. This is because the cover slip on the sample and air have different refractory indexes. The light scatter that occurs during the transition from glass to air is noticable at such a high magnification. Immersion oil has a refractive index very similar to the cover slip, thus reducing the light scatter as the light passes from the sample to the objective lens.
What is the second lens in a traditional refractor called?
Refracting telescopes use a main lens made from glass and called the objective lens.
What is the second lens ina traditional refractor called?
Refracting telescopes use a main lens made from glass and called the objective lens.
What objective lens is it easiest to find or locate your specimen?
You should use the lowest value lens. It is the shortest one.
Why is it necessary to use emmersion oil with the 100x objective?
because at this magnification the light diffraction in air is to important to have enough light reach the lens, so oil should be in the contact between the slide and the lens because it has a much lower refraction index than air allowing more light to reach the lens
How do you remove oil from the slide and oil immerssion lens after use?
When you desire to remove oil from a slide and an immersion lens after using, for example, cedar oil, you would use Xylene to remove the oil. Natural oils can harden on the lens otherwise.
When do you use the high power objective?
The high power objective on a microscope is used anytime you need to achieve greater detail and magnification, and is often used when identifying cells. The high power objective is generally not used in teaching/learning labs as it is usually an oil immersion lens. Using oil means greater cleaning & care is required.
