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If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.

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Q: Why do you use on or slide to be examined with the oil immersion objective?
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What objective focuses closest to slide?

The oil immersion lens @100x


Which objective focuses closest to the slide when it is in focus?

The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.


When using the oil immersion lens you should raise the objective lenses for easy application of an oil droplet to the slide is this true or false?

False


Which objectives focuses closest to the slide?

oil immersion


Oil immersion is used as a medium between the specimen and the oil immersion objective lens why?

Oil immersion is a technique used to increase the resolution of a microscope.


What microscope objective is best to study bacteria?

The most satisfactory way to study bacterial morphology 40x objective.


When would you need to use the oil immersion lens?

Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.


Why should you never look at a wet mount slide with a oil immersion lens?

Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.


What micrscope objective is best to study bacteria?

100x with oil immersion


Why should the oil immersion objective be cleaned immediately after use?

Because the oil will dry and distort the refraction of the light through the objective.


What is OIO in microscope?

oIO, stands for oil immersion objective. it was discovered during 1870. the OIO, magnifies the actual size of the specimen , 100 times, with the aid of placing a drop of ceddar oil at the top of the coverslip in slide. Remember that the OIO objective must touch the surface of the oil.


What are the advantages of the oil immersion objective?

One advantage is that it increases the resolution and clarity of the image, this is very useful for viewing specimens that would otherwise be to small to see without the oil. However it can be very messy to apply the oil and can take time and be rather difficult to clean the slide and the microscope. Moreover the slide could become damaged if vertical movement is used a lot; this is because the work distance between lens and slide is very small.