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What would happen if electrodes were plugged into wrong outlets during gel electrophoresis?
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When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
What is the Working principal of electrophoresis?
Separates DNA fragments so they can be seen
What are inert and active electrodes?
Inert electrodes simply serve as electrical conductors and are unchanged by the cell processes. Active electrodes change during the cell reactions. An example of inert electrodes is platinum in the electrolysis of water. The platinum remains unchanged and the water is split into hydrogen and oxygen. An example of active electrodes is in the electrolysis of copper sulfate solution with copper electrodes. The anode copper is converted into copper ions in the solution, and metallic copper builds up on the cathode. The term active electrode is also used in eeg measurement, here as the opposite to passive electrode. Electrodes are the pads attached to the skin to get readings, and active ones don't need a conductive paste to get a good signal.
What precaution should take during gel electrophoresis?
when using the micropipettes,be careful and avoid contaiinating the micropipettes by slowly releasing the plunger
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
Which fragements travel the fastest and farthest during electrophoresis?
The smallest and lightest fragments.
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
What are ignition electrodes on boilers?
IGNITION ELECTRODES IMPLIES TWO ARE INSTALLED. TWO ELECTRODES IMPLIES AN OIL FIRED BOILER. THE ELECTRODES ARE TIED ELECTRICALLY TO THE SECONDARY WINDINGS OF A HIGH VOLTAGE TRANSFORMER. DURING IGNITION THE TRANSFORMER PRODUCES A SPARK BETWEEN THE ELECTRODES TO IGNITE THE ATOMIZED MIXTURE THAT THE BURNER MOTOR AND OIL PUMP PRODUCE AND DELIVER VIA THE NOZZLE. LC
Can always keeping pc plugged in during non use damage my computer?
Yes, by keeping the PC plugged in during the non-use can really damage the computer.
What is the Working principal of electrophoresis?
Separates DNA fragments so they can be seen
What is used in electrodes in car batteries?
In traditional "lead-acid" batteries, the electrodes are lead and lead (IV) oxide. During discharge, both electrodes dissolve in the strong acid electrolyte to form dissolved lead (II) cations. During recharge, these reactions are reversed. Other battery types, with a longer rechargeable life are used for hybrid electric-gasoline cars.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
Properties of DNA fragments which allow them to be separated from each other during gel electrophoresis?
They are negatively charged and are of different sizes
What is the proper placement of EKG electrodes during cardiac angiography?
please display a diagram of lead placement
Why electrodes get red hot during welding?
They shouldn't. When using the correct electrode and amperage they will get hot but not red hot. Uncoated electrodes do get red hot but are not used in most applications. Too much amperage is usually the problem.
What are inert and active electrodes?
Inert electrodes simply serve as electrical conductors and are unchanged by the cell processes. Active electrodes change during the cell reactions. An example of inert electrodes is platinum in the electrolysis of water. The platinum remains unchanged and the water is split into hydrogen and oxygen. An example of active electrodes is in the electrolysis of copper sulfate solution with copper electrodes. The anode copper is converted into copper ions in the solution, and metallic copper builds up on the cathode. The term active electrode is also used in eeg measurement, here as the opposite to passive electrode. Electrodes are the pads attached to the skin to get readings, and active ones don't need a conductive paste to get a good signal.
