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Difference between genomic library and cdna library?
A cDNA (complementary DNA) library is a DNA library that has been created from mRNAs that are present in the cell. Since a cDNA is created from mRNA transcripts, that means that in Eukaryotic organisms there will be no introns or transcriptional factors present in the cDNA library, only exons. Only protein coding regions will be present in a cDNA library. This also means that a cDNA library is often times tissue specific. Since the expression of mRNAs will be different in different tissues of the organism it will appear different then a genomic library. Often times to offset this problem a cDNA library will be composed of different tissues (brain, liver, heart) to encompass a greater variety of the proteins that are expressed. A genomic library will contain all the exons, introns, and transcriptional factors that are not found in the cDNA library. **2/24/2011** cDNA library does contain exons, which is the protein coding regions.
What is the difference between Plasmic DNA and Genomic DNA?
Plasmic DNA is extrachromosomal DNA found in prokaryotes that replicates independently of the chromosomal DNA. Genomic DNA refers to the complete set of genetic material within the nucleus of eukaryotic cells or the nucleoid region of prokaryotic cells.
What is genomic region?
A genomic region refers to a specific part of a genome that may contain one or more genes along with regulatory elements. It can vary in size and is typically defined by its unique DNA sequence. Researchers often study genomic regions to understand their functions and the genetic variations present within them.
What is a map of the distribution of cloned genomic DNA from genomic clone libraries?
A map of the distribution of cloned genomic DNA from genomic clone libraries shows the physical location of different DNA fragments within the genome. This type of map is used to study genetic organization, identify genes, and analyze specific regions of interest within the genome. It helps researchers understand the genomic structure and function of an organism.
How much percentage of genomic DNA is active?
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How many strands of DNA are there in a DNA fingerprint?
1 strand of naked genomic DNA cut by certain enzymes.
Why do you use ethanol after chloroform and isoamylalcohol mixture and isopropanol during genomic DNA isolation?
Ethanol is used after the chloroform and isoamylalcohol mixture to precipitate DNA from the solution. Isopropanol is used during genomic DNA isolation to further facilitate the precipitation of DNA, ensuring a higher yield and purity of DNA in the final step.
Why mitochondria mutation study will isolated Genomic DNA instead of Mitochondrial DNA?
Yes,I don't know!Tell me..!
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
What is meant by the term 'genomic DNA'?
Geonomic DNA references to the genome in the structure of the the DNA. The genome in the DNA is what makes people unique and without it, everyone would be the same.
What are the recommended steps for using a gdna extraction kit for isolating genomic DNA from a sample?
Prepare the sample by collecting and processing it according to the kit's instructions. Follow the kit's protocol for cell lysis to release the genomic DNA. Add the necessary reagents and enzymes to the sample to purify the DNA. Use the provided columns or beads to separate the DNA from other cellular components. Wash the DNA to remove any contaminants. Elute the purified genomic DNA from the columns or beads using a suitable buffer. Store the extracted DNA properly for future use.
What does RNase solution do in the Isolation of genomic DNA from Negative bacteria?
Digests RNA molecules
