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How much percentage of genomic DNA is active?
kk
What is a map of the distribution of cloned genomic DNA from genomic clone libraries?
A map of the distribution of cloned genomic DNA from genomic clone libraries shows the physical location of different DNA fragments within the genome. This type of map is used to study genetic organization, identify genes, and analyze specific regions of interest within the genome. It helps researchers understand the genomic structure and function of an organism.
Why do we incubate during extraction of genomic DNA?
Incubation during the extraction of genomic DNA is crucial for several reasons. It allows for the lysis of cells and the release of cellular components, including DNA, by breaking down cell membranes with lysis buffers. Additionally, incubation at specific temperatures can enhance the activity of enzymes, such as proteases and nucleases, which help to digest proteins and other contaminants that may interfere with DNA isolation. This process ultimately leads to a purer and more intact genomic DNA sample.
What will be the positions of genomic DNA plasmid DNA and RNA in the agarose gel electrophoresis?
RNA band will be near the wells being single stranded,Genomic DNA will be at the centre of the gel being linear double stranded and plasmid DNA being circular moves faster and will be the brightest hence will be near the base..
What is the difference between plasmid and episomes?
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The only difference between the plasmid and the episome is the integration i-e plasmid can not integrate while episome can integrate into the genome.
Where does genomic DNA present in a tissue?
In the mitochondria
How much percentage of genomic DNA is active?
kk
What is a map of the distribution of cloned genomic DNA from genomic clone libraries?
A map of the distribution of cloned genomic DNA from genomic clone libraries shows the physical location of different DNA fragments within the genome. This type of map is used to study genetic organization, identify genes, and analyze specific regions of interest within the genome. It helps researchers understand the genomic structure and function of an organism.
Difference between genomic library and cdna library?
A cDNA (complementary DNA) library is a DNA library that has been created from mRNAs that are present in the cell. Since a cDNA is created from mRNA transcripts, that means that in Eukaryotic organisms there will be no introns or transcriptional factors present in the cDNA library, only exons. Only protein coding regions will be present in a cDNA library. This also means that a cDNA library is often times tissue specific. Since the expression of mRNAs will be different in different tissues of the organism it will appear different then a genomic library. Often times to offset this problem a cDNA library will be composed of different tissues (brain, liver, heart) to encompass a greater variety of the proteins that are expressed. A genomic library will contain all the exons, introns, and transcriptional factors that are not found in the cDNA library. **2/24/2011** cDNA library does contain exons, which is the protein coding regions.
How many strands of DNA are there in a DNA fingerprint?
1 strand of naked genomic DNA cut by certain enzymes.
What is the difference between genomic and bioinformatics?
genomics is the study of an entire sequence of an organism's DNA, while bioinformatics is the use of computers and data bases to organize and analyze DNA. bioinformatics makes genomics a bit easier.
Why do you use ethanol after chloroform and isoamylalcohol mixture and isopropanol during genomic DNA isolation?
Ethanol is used after the chloroform and isoamylalcohol mixture to precipitate DNA from the solution. Isopropanol is used during genomic DNA isolation to further facilitate the precipitation of DNA, ensuring a higher yield and purity of DNA in the final step.
What is the relationship between methylation and genomic imprinting?
Methylation is a process where methyl groups are added to DNA, which can affect gene expression. Genomic imprinting is a phenomenon where certain genes are expressed based on whether they are inherited from the mother or father. Methylation plays a key role in genomic imprinting by regulating which parent's gene is expressed.
Why mitochondria mutation study will isolated Genomic DNA instead of Mitochondrial DNA?
Yes,I don't know!Tell me..!
Difference between the DNA and RNA?
the difference is that DNA is a double helix and RNA is a single chain
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
What is meant by the term 'genomic DNA'?
Geonomic DNA references to the genome in the structure of the the DNA. The genome in the DNA is what makes people unique and without it, everyone would be the same.
