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DNA Splitting
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∙ 11y ago
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Are restriction endonucleases obtained from various species of bacteria?
Yes
What do biotechnologist used to cut DNA molecules at specific sequences?
Restriction endonucleases or enzymes
What type of the enzyme that cuts the bonds of DNA molecule at the origin of replication?
restriction endonucleases
What is meant by the term restriction digest?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
What is the cutting of DNA by specific enzymes known as restriction endonucleases the cut piece from one organism is added to the DNA of another?
GE
What is the difference between Restriction endonucleases and exonucleases?
Exonuclease enzymes cleave nucleotides from the ends of DNA molecules. Endonuclease enzymes cleave a phosphodiester bond somewhere within the DNA molecule (not at the ends).
Explain the Molecular mechanism functions of restriction endonucleases?
Restriction Endonucleases recognize certain sites on the DNA or the sequences. For example EcoR1 that recognizes the restriction site GAATTC on any strand of DNA or RNA.
What causes DNA to become fragmented?
restriction endonucleases.
Are restriction endonucleases obtained from various species of bacteria?
Yes
What do biotechnologist used to cut DNA molecules at specific sequences?
Restriction endonucleases or enzymes
What do biotechnologists use to DNA molecules at specific sequences?
Restriction endonucleases or enzymes
What type of the enzyme that cuts the bonds of DNA molecule at the origin of replication?
restriction endonucleases
What is meant by the term restriction?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
What is meant by the term restriction digest?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
EcoRI and HindIII are What?
They are type II restriction enzymes with a ommon structural core comprising four β-sheets and a single α-helix. restriction endonucleases
What is the cutting of DNA by specific enzymes known as restriction endonucleases the cut piece from one organism is added to the DNA of another?
GE
What cuts bacterial plasmids?
Enzymes called restriction endonucleases can cut plasmids. However, in order for a cut to be produced, the plasmid should contain a specific sequence of nucleotides called the restriction site
