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Which component is used to differentiate between organisms in DNases agar?
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Which enzyme opens DNA molecules?
Restriction Enzymes
Why ph8 is maintained during isolation of plasmid?
EDTA is dissolved only at pH8. EDTA serves as an important chelating agent to kill the contaminating DNAses. Also this is close to the physoological pH which is pH7.
What is the difference between Clean-room and sterile-room?
You may want to double-check this, but I believe PCR-clean simply means that there are no DNases or RNases on the item, but they still could have nucleic acid on them. Essentially there is nothing on them that would interfere with nucleic acid amplification achieved with PCR, but any genetic material they may have will be amplified. Sterile means that there is absolutely no genetic material on the item itself (usually achieved via autoclaving where the temperatures climb so high that they would denature the DNases and RNases anyway). Nutshell: PCR-clean = wiping with RNA away and DNA away Sterile = bleaching and autoclaving
Role of TEG buffer in alkali lysis?
Glucose is added to increase the osmotic pressure outside the cells. Tris is a buffering agent used to maintain a constant pH ( = 8.0). EDTA protects the DNA from degradative enzymes (called DNAses); EDTA binds divalent cations that are necessary for DNAse activity.
What did Avery's theory provide evidence for?
Ostwald T. Avery's experiment was an extension of the works of Griffith's. Griffith's experiment using virulent and avirulent Streptococcus pneumoniae (which had different colony characteristics) proved that it was possible for the heat killed virulent Streptococcus pneumoniae to pass on the genetic information for formation of smooth colonies to avirulent strains. Avery, Mc Leod and Mc Carty conclusively found out that the genetic material responsible for transfer of characteristics between the strains was DNA. This was achieved by incubating the cell lysate of virulent Streptococcus with proteases, RNAses and DNAses separately before mixing it with avirulent strains. It was found that only when the lysate was incubated with DNAses did the transfer of smooth colony formation characteristic from the virulent strain to the avirulent strain fail to occur.
What is the function of TEB buffer in gel electrophoresis?
Restriction buffer maintains the pH in a range suitable for enzyme activity, as well as supplying salt cofactors required for catalysis. Since different restriction enzymes require varying salt conditions and pH, a single compromise buffer can be used that strikes a balance between conditions preferred by the various restriction enzymes. (Spec. compromise restriction buffer)
What is the function of Tris cl in extraction of DNA?
TRIS maintains the pH of the solution. Basically it interacts with the lipopolysaccharides present on the outer membrane which helps to permeabilize the membrane. This effect is enhanced with the addition of EDTA.
What cuts DNA into fragments?
cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.
What is the role of protinase K in DNA isolation?
I believe the role of proteinase K in a DNA isolation is just to digest proteins. Proteinase K is a protein digesting enzyme. Digesting proteins is important in a DNA isolation because the proteins included in your DNA before you treat it with proK likely include some DNAses. If you didn't use proK, your DNA would degrade very quickly.
How does hydrogen affect your life?
With respect to chemistry you may say that Life depends upon hydrogen bonding. how ? life is created from water and water is a liquid due to hydrogen bonding in its molecules, otherwise water must be a gas just like air, (no oceans, no lakes no rivers), the fluids in cells of living bodies also exist due to hydrogen bonding (among organic molecules) so all the living systems depend upon hydrogen bonding.
Function of SDS in DNA isolation?
SDS is used as a lysing solution because is a detergent so it dissolves the cell membrane which is made out of lipids, but is also used to denature proteins once the cell has been lysed, i hope this helps
