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Which enzymes do scientists use to bind a new gene to plasmid DNA?
Wiki User
∙ 7y ago
The enzymes to join DNA fragments are called ligases. Two of the most common are: 1) T4 DNA ligase (from bacteriophage T4), this enzyme, a single polypeptide of Mr = 68 kDa, catalyses the formation of a phosphodiester bond between adjacent 3'-OH and 5'-P termini in DNA; and 2) T4 RNA ligase, that catalyzes the covalent joining of 5'-phosphoryl, single stranded DNA or RNA to 3'-hydroxyl, single stranded DNA or RNA. T4 RNA ligase increases the efficiency of blunt-end ligation of double-stranded DNA catalyzed by T4 DNA ligase.
Wiki User
∙ 7y ago
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Which enzyme do scientists use to bond a new gene to plasmid DNA?
DNA ligase
What are the two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA?
HinD III and Bam HI
What are the genes on the pVIB plasmid?
It contains a gene for luciferase, a Lux gene (the enzyme that catalyzes the light-emitting reaction) and genes for enzymes which produce the luciferins (which are the substrates for the light-emitting reaction.). This causes bacterial cells to glow!
What does a repressor do in the enzymes active site?
Repressors bind to the silencers in the DNA to block the RNA polymerase from binding to the promoter of the gene to reduce gene expression, not really binding to enzymes active sites I think what you meant was "what does an inhibitor do to the enzymes active site"? In which case, it depends on the type of inhibitor. A competitive inhibitor has a structure similar to the substrate, hence would bind to the active site as well, competing with the substrate for the enzyme active sites, decreasing enzymatic activity. A non-competitive inhibitor binds to the allosteric site of the enzyme, causing a structural change in the enzyme active site shape. Hence the enzyme would not be able to bind to the original substrate, so enzymatic activity comes to a halt for the enzymes that are bound by the non-competitive inhibitors
In the process of human gene cloning using recombinant plasmids what is the bacterial plasmid?
functions as a vector
A scientist wants to insert new gene into a bacterium what is the first step in this process?
cutting the gene out of the DNA with enzymes
Which enzyme do scientists use to bond a new gene to a plasmid?
DNA ligase
Which enzyme do scientists use to bond a new gene to plasmid DNA?
DNA ligase
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
What are the two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA?
HinD III and Bam HI
What are the genes on the pVIB plasmid?
It contains a gene for luciferase, a Lux gene (the enzyme that catalyzes the light-emitting reaction) and genes for enzymes which produce the luciferins (which are the substrates for the light-emitting reaction.). This causes bacterial cells to glow!
What would be the advantages of inserting into a plasmid gene with a highly visible phenotype?
Inserting a plasmid gene into the organism gives us three situation that one is the foreign cell may not pick up the plasmid the second chance is it is picked up may not expressed and in the third case it is expressed and therefore you can have the gene of interest. This is the one main advantage of studying the gene of interest by inserting a plasmid gene.
Why is ampicillin added to the pound medium?
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means no plasmid in the bacterial host...
Why must scientists use only certain enzymes when inserting or removing a defective gene from a cell?
Explain
Which enzyme would cut the plasmid without disrupting the function of?
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
How does a human insulin genes become part of a plasmid?
1. Scientists remove plasmids, small rings of DNA, from bacterial cells. 2. An enzyme cuts open the plasmid DNA. The same enzyme removes the human insulin gene from its chromosome. 3. The human insulin gene attaches the open ends of the plasmid to form a closed ring. 4. Some bacterial cells take up the plasmids that have the insulin gene. 5. When cells reproduce, the news cells will contain copies of the engineered plasmid. The foreign gene directs the cell to produce human insulin.
What is the methodology for producing recombinant DNA to be used in gene cloning?
1. A vector such as plasmid is needed along with a host cell. Restriction enzymes and DNA ligase are enzymes that are used to introduce foreign DNA into a vector.
