Gram + bacteria do NOT grow on EMB agar, EMB agar inhibits their growth, and is selective for Gram -
both selective and differential grow
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MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
it inhibits the growth of Gram positive bacteria
This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
No. A treatment of ethylenediaminetetraacetic acid (EDTA) and the enzyme, lysozyme, will cause gram negative bacteria to lyse. Some gram positive bacteria can also be lysed by doing this. The EDTA stabilizes the solution and allows lysozyme to cut the bonds between the carbohydrates that make up the cell wall wall in bacteria. This will lyse the cell and cause the contents of the cell to be spilled out. This technique is primarily used for DNA assays. A good way to isolate either gram positive or gram negative is by using different types of agar. The MacConkey agarmight be the most useful for what you want to do. It will only allow gram negative to grow, isolating just the gram negatives only.
yes It can, because MSA grows gram positive bacteria.
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
There is no growth because S.Epidermidis is a gram positive bacteria. MacConkey selects for gram negatives. the bile salts and crystal violet inhibit gram positive growth in the medium
it inhibits the growth of Gram positive bacteria
MacConkey's agar refers to a culture medium that is designed to selectively grow Gram-negative bacteria. It contains crystal violet dye, which inhibits certain Gram-positive bacteria.
gram positive bacteria cannot grow due to the presence of bile salts and crystal violet in maConkey media.
Macconkey agar consists of agar, lactose, peptone, neutral red dye, crystal violet dye & bile salts. Bile salts inhibit growth of Gram positive bacteria & allow growth of Gram negative bacteria only. It differentiates lactose fermentating Gram negative bacteria from non lactose fermentating Gram negative bacteria.
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This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
No. A treatment of ethylenediaminetetraacetic acid (EDTA) and the enzyme, lysozyme, will cause gram negative bacteria to lyse. Some gram positive bacteria can also be lysed by doing this. The EDTA stabilizes the solution and allows lysozyme to cut the bonds between the carbohydrates that make up the cell wall wall in bacteria. This will lyse the cell and cause the contents of the cell to be spilled out. This technique is primarily used for DNA assays. A good way to isolate either gram positive or gram negative is by using different types of agar. The MacConkey agarmight be the most useful for what you want to do. It will only allow gram negative to grow, isolating just the gram negatives only.
yes It can, because MSA grows gram positive bacteria.
Scanning about the microbiologic community, a few references to Gram pos bacteria growing on MacConkey's showed up. One reports a Gram positive rod of unspecified species (see links). Another article says some forms of Enterococcus and Staphylococcus can grow in spite of the bile salts that make this medium so antagonistic to Gram positives. But just as many articles opine that, if this happens, the medium may not have been properly prepared.
Gram-positive bacteria grows in Mannitol agar because it contains a high level of salt. This type of agar allows only certain types of bacteria to grow, making it selective.Ê