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What bacteria is used in acid-fast staining?
It is used for those bacteria which contain fat or lipid layer on their outer wall, and did not stain with grams staining. e.g. Mycobacterium
Which is the Counterstain used in PAS staining?
The counterstain for PAS is hematoxylin which stains nucleic acids blue.
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
What is staining rack?
Staining rack is used to hold many glass slides at a time. By putting slides on a staining rack, you can pour dye simultaneously and it will help to reduce extra dye or stain.
Ziehl-Neelson staining procedures is used for detection?
Franz Ziehl(Bacteriologist) andFrederick Neelson(pathologist) are the two scientists who discovered Ziehl-Neelson stain or acid-fast stain.Mycobacterium tuberculosis is an organism with high lipid conent in its cell wall because of which acid fast stain is used. 1.smearpreparation 2.flood with cabolfuchsin under continuous heating 3.wash with acid alcohol(20%hci:ethanol) 4.flood methelene blue.wash with water observe under microscope
What is secondary stain?
A secondary stain is Methylene blue. This type of stain is used in a acid fast staining. This type of staining test can determine medical conditions such as tuberculosis.
What are the consequences of Ziehl-Neelsen staining?
The Ziehl-Neelsen stain is also known as the acid-fast stain. It contains sulfuric acid, and is used to identify acid-fast bacteria, or bacteria resistant to decolorization by acids from staining.
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
What so endospore stain have in common with the acid-fast stain?
One thing that endospore stains have in common with the acid fast stain is that heat primary stain penetration. Another thing that endospore stains have in common with acid fast stains are counterstain.
Does green tea stain your teeth?
Believe it or not, tannic acid. And Tannic acid is also used in the chemical staining of wood, so if it stains wood, it'll stain your teeth
What is an acid-fast pathogen?
An acid-fast pathogen is a bacteria that is harmful to humans. They have cell walls that contain mycolic acid which is a lipid. Common Gram type staining techniques wont work with these cells. A special stain carbolfuchsin is used to penetrate the wall.After staining you wash with acid alcohol if the stain remains it is acid fast if it washes out it is non-acid fast.Mycobacterium tuberculosis is a well known acid-fast pathogen
What bacteria is used in acid-fast staining?
It is used for those bacteria which contain fat or lipid layer on their outer wall, and did not stain with grams staining. e.g. Mycobacterium
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
Why nucleic acid is called nucleic acid?
Because it is used in the nucleus of a cell.
Is a Gram stain an adequate substitute for an acid fast stain?
So few organisms are acid-fast, the acid fast stain is used only when infection by an acid-fast organisms is suspected.
Why do you use acid fast stain?
Because it is a type of stain that uses contrasting (in this case the presence or absence of a stain) color to determine what type or classification the organism is. Differential = determining between different groups; you are staining to differentiate one group from another by classifying the organisms' ability to quickly resist decolorization by acids.
