Hershey and chase
The scientists used a blender to break open the bacterial cells infected by phages, releasing the phage DNA. This demonstrated that the phage DNA carries instructions to produce new phage particles when introduced into a host bacterial cell.
The substance a phage leaves outside its host cell is typically referred to as the "phage progeny" or "phage particles." These particles can include newly synthesized phage DNA and proteins, as well as the phage capsid that encapsulates the genetic material. This material can go on to infect other host cells and continue the phage replication cycle.
Phage titration is needed to determine the concentration or titer of phages in a sample, which is crucial for ensuring the effectiveness of phage-based therapies, determining the optimal phage concentration for experiments, and monitoring phage growth dynamics in culture. Titration allows researchers to quantify the number of active phages present in a sample, providing valuable information for various applications in phage research and biotechnology.
A phage infects bacterial cells by injecting its genetic material into the host bacterium. Once inside, the phage hijacks the bacterium's cellular machinery to replicate and produce more phage particles, ultimately leading to the bacterium's destruction.
Bacillus anthracis is susceptible to gamma phage because the phage has evolved to target and infect specific receptors on the surface of the bacterium. This interaction enables the gamma phage to inject its genetic material into the bacterium, hijacking its machinery to produce more phage particles and ultimately leading to the destruction of the bacterial cell.
They used the T2 phage in their Blender experiment.
In the lytic cycle, a phage kills the bacterial host cell by causing it to burst (lysis) to release new phage particles. The phage replicates inside the host cell until it reaches a critical point, then the host cell is ruptured to release the new phages to infect other host cells.
phage
temperate phage
DNA single
c. Repression of the phage genome - A phage coded protein, called a repressor, is made which binds to a particular site on the phage DNA, called the operator, and shuts off transcription of most phage genes EXCEPT the repressor gene. The result is a stable repressed phage genome which is integrated into the host chromosome. Each temperate phage will only repress its own DNA and not that from other phage, so that repression is very specific (immunity to superinfection with the same phage).Reference: http://pathmicro.med.sc.edu/mayer/phage.htm
Phages insert their genetic material, which is typically DNA, into bacteria. This genetic material carries the instructions for the phage to replicate itself within the bacterial cell.