Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
To remove unwanted microorganisms, dust particles and grease etc.....
size and shape
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
flagellar stain
not sure
If you used acid-alcohol as the decolorizing agent in spore staining the cells of the bacillus are gram plus. If you use acid alcohol it will not decolorize gram plus cell wall as only gram negative are decolorized. It will not get a differentiation in color.
Heat is the mordant used in the spore stain, it fixes the primary stain.
Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
Gram staining: This is to determine if a bacterial cell is Gram positive or negative. This uses Crystal violet dye, Gram's iodine as a mordant, Ethyl Alcohol as a decolorization medium, and Safranin as a secondary dye. Spore staining: Primary dye is Malachite green, then slide is placed over boiling beaker, cooled, rinsed with water, then Safranin is used as a counter stain. This test is used to show whether a bacteria is a spore former. Acid fast staining: Primary dye is Carbolfuchsin, heated over beaker like the spore stain, acid alcohol is used as the decolorizing agent, and Methylyene Blue is used as a counter stain. This is used to show bacteria with acid-fast walls, which have a thick waxy lipid around them. These are the most commonly used staining techniques with Bacteria.
Enter a double-battle with smeargle and a Pokemon with spore. Have that Pokemon use spore and have smeargle use sketch on him/her.
Any good alcohol or lacquer based sealer will work. I use shellac if I'm staining and Kilz if I'm painting.
Out of what exactly? The method depends on the fabric it is staining. You can use hairspray, rubbing alcohol or milk, depending on exactly what the fabric is.
alcohol is organic solvent , it dissolves lipid in cell wall of Gram negative bacteria . this allows out flow of C.V. and counter staining by safranine which results in red color of Gram negative bacteria .
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
The spore would appear to be red as the safranin is heat driven into the many layers of the spore, however, as Malachite green has a weak affinity and is water soluble, it will not likely bind to the spore wall or the cell wall. You might have traces of green on the slide if any, but it will be very little. Your vegetative cells will be pink as well.
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.