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Why are DNA samples and restriction enzymes incubated for 5 minutes?
ObanlaTemitayo
uranes then myanes
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∙ 11y ago
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How are restriction enzymes used to look for differences between DNA samples?
Restriction enzymes cleave, or open, the DNA so that a sample can be taken and gel electrophoresis can separate the strands of DNA. From there, DNA probes bind to certain strands in each sample and DNA fingerprints can show the differences.
How do you use electrophoresis to determine paternity of a baby?
Children receive half of their genetic material from each parent. There are specific sites on DNA, known as restriction sites, that are recognized by restriction enzymes. These are used to determine paternity. Samples of DNA from the mother, father and child are taken. They are all digested ('cut') by the same restriction enzymes. These DNA fragments are then separated by gel electrophoresis (which separates fragments based on size). The bands of the child are compared to the mother and father's. If the band is not the same as the mother's, it must have come from the father. If these do not match up, then the sample was not taken from the biological father.
What does the formation of recombinant DNA result from?
Recombinant DNATo to make recombinant DNA or plasmids, the two different samples of DNA need to be cut up by the same restriction enzyme. Restriction enzymes cut DNA at specific sequences (restriction sites) and is usually a staggered cut. For example, say you had the following sequence of DNA (both strands): 5' GAATTC 3'3' CTTAAG 5'Say the restriction enzyme used will cut a strand between a guanine and adenine on one strand and an adenine and guanine one the other strand. For the given DNA, there would be cuts where the bars are:5' G|AATTC 3'3' CTTAA|G 5'Then the strands would separate:5' G--------AATTC 3'3' CTTAA--------G 5'Because the cuts are staggered, hydrogen bonds are left open. The ends of the restriction fragments are called "sticky ends" because of their ability to bond to other fragments. Remember that both sets of DNA are cut with the same restriction enzyme. Therefore, the sticky ends of the restriction fragments are complementary to each other. Then you're able to take one fragment of one DNA sample and insert it into the other DNA sample, which are bound together by hydrogen bonds. DNA ligase is then added to seal the ends together.
Which type of samples analysed by hplc?
The samples that are not volatile and are soluble in the mobile phase.
Holes drilled several kilometers into earth's crust provide direct evidence about earth's interior in the form of?
Rock Samples.
How are restriction enzymes used to look for differences between DNA samples?
Restriction enzymes cleave, or open, the DNA so that a sample can be taken and gel electrophoresis can separate the strands of DNA. From there, DNA probes bind to certain strands in each sample and DNA fingerprints can show the differences.
DNA strands can be clipped crosswise at selected positions by using enzymes called?
DNA can be cut into smaller fragments by enzymes (which are proteins) known as restriction endonucleases (REN's). These enzymes are sequence specific - meaning they produce a cut only at a particular site on the DNA strand. This site where the cut is produced is called the restriction site. Restriction sites are 4 - 6 nucleotides in length. Every restriction enzyme has a different restriction site. This property allows researchers to treat two different DNA samples with the same set of restriction enzymes and then analyze the resulting fragments.A. DNA finger printing
How do you use electrophoresis to determine paternity of a baby?
Children receive half of their genetic material from each parent. There are specific sites on DNA, known as restriction sites, that are recognized by restriction enzymes. These are used to determine paternity. Samples of DNA from the mother, father and child are taken. They are all digested ('cut') by the same restriction enzymes. These DNA fragments are then separated by gel electrophoresis (which separates fragments based on size). The bands of the child are compared to the mother and father's. If the band is not the same as the mother's, it must have come from the father. If these do not match up, then the sample was not taken from the biological father.
Why is DNA fingerprinting more accurate if the samples are cut with more than one restriction enzyme?
When EcoR1 cuts this DNA, it cuts it at three places into four different segments. EcoR1 is only one of many different restriction enzymes. Each different enzyme cuts DNA at a different site. By using different enzymes, a scientist can cut DNA into many smaller pieces that can be run out on a gel during electrophoresis. Remember that in gel electrophoresis, DNA fragments separate by size. Because these segments have different sizes, they will separate onto a gel at different rates. If different people's DNA is cut by restriction enzymes and then run out on a gel, each person's DNA will leave a different pattern.
How long are Blood samples good for testing?
Blood samples can be held in storage for 17 to 180 days. The length of their storage time depends on which enzymes have been added to the sample.
What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
Why do you think the DNA is storeed both cold and with the InstaGence matrix affter boiling the samples?
any heat can activate the DNA- degrading enzymes
How long are blood tests good for?
Blood samples can be held in storage for 17 to 180 days. The length of their storage time depends on which enzymes have been added to the sample.
What is an Omnitrix?
an omnitrix is a device which stores dna samples. it is like a wristwatch which can turn you into an alien for 10 minutes. it is fictional and does NOT.
Why is it helpful to digest each of your samples with two different restriction enzymes?
It helps break up your sample even more. Your first enzyme may, for example, ONLY cut at a sequence of TAATTA ---> TA // ATTA. Maybe your second enzyme is less selective, and will cut ANY GC --> G // C. Using them together, you will end up with much smaller fragments than using only enzyme #1 for example.
Do i say can i have samples about them or samples of them?
samples of them
How much storage is require for 72 minutes for 24 bit 96kHz stereo audio?
96KHz implies 96000 samples/second. 24 bits is 3 bytes a sample. That would be 0.096 Million samples/second x 3bytes/sample x 60(seconds) x 72(minutes) = 1.24GB of raw data if you captured it as wav.
