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Maybe because bananas are annual kind of plant, which means they can survive in all year round in both rainy season and summer season and it's easy to produce unlike to the other plant to our surroundings. On the other hand, bananas have a juicy stem or body, it has a complete structures of genetic information and besides banana can reproduce itself, without mating from the other plant nor from the other species of banana.(asexual reproduction).
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What does sodium citrate do in DNA extraction?
how to make sodium citrate in 10% ethanol for DNA extraction
What are the precaution while conducting DNA extraction?
Perhaps the most basic precaution is to make sure that the warm water bath that will be used has a temperature that doesn't exceed 90C so as to denature only the protein component of crude extract and not the DNA which is the target isolate.
What are the different methods of DNA and RNA extraction?
DNA extraction is done by three methods: * Organic extraction * inorganic extraction * solid state method In organic extraction, phenol and chloroform are used to create on organic phase in which cells are lysed and DNA is freed. The DNA remains in the aqueous phase. Ethyl alcohol is used to precipitate the DNA. In the inroganic methos, NaCl and EDTA are used for cell lysis. Following this, an approach similar to the organic method is followed. In solid state extraction, DNA is first precipitated in the presence of high slat and low pH conditions. The precipitated DNA is then adsorbed on to a filter membrane surface.
What is the function of liquid detergent in genomic DNA extraction?
Liquid detergent used in the genomic DNA extraction, emulsify plasma membrane and nuclear membrane promoting lysis. SDS (Sodium Dodecyl Sulphate) is an anionic detergent used in DNA extraction. It removes the positive ions from the proteins, due to this protein loses its conformation and gets destroyed thus the cell membrane gets damaged and cell gets broken.
What is Buffer ATL?
Buffer ATL is a proprietary chemical used for DNA extraction and purification. It's used to induce lysis of cell tissues in order to release and expose the cell's DNA during the beginning stages of of the extraction process. I think the letters "ATL" stand for "Animal Tissue Lysis." It's part of the DNeasy protocol from Qiagen. I use this every day during my extraction and purification of DNA from various snake species.
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
Is DNA extraction used in cloning?
yes
Why extraction buffer is added for isolation of DNA?
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
What mesh particle size for chelex 100 is best for DNA extraction?
The 200-400 mesh size is best for DNA extraction. The smaller sizes are usually used for metal ion extraction.
What temperature is used to speed the extraction of DNA?
55-60oC
What is the function of tritonx100 in DNA extraction?
Triton X-100 is used as a lysis buffer for DNA separation.
Why is buffer HB used in DNA extraction?
It serves to break the tissue apart so the DNA can be subsequently extracted.
Why saturated KCL in DNA extraction is used?
Saturated KCl precipitation is often used in DNA extraction for molluscan taxa. Molluscs produce a polysaccharide rich mucus that interferes with the reagents involved in DNA extraction. The KCl saturated solution is used right after the digestion step: about 1/4th of the volume of the digestion solution is added to the sample. Samples are then centrifugated at 14rpm for 15 minutes. The pellet formed will contain the polysaccharides and non digested tissue. The supernatant is extracted from the tube and used in the next steps of the DNA extraction.
What is the point of mashing straberrys for DNA extraction?
Use the usual extraction protocol for plants: grind, liquid-liquid extraction, salting out.
What does sodium citrate do in DNA extraction?
how to make sodium citrate in 10% ethanol for DNA extraction
DNA extraction from bacteria?
DNA extraction from bacteria can be achieved in various ways. Yeast is the best resource to extract the DNA bacteria from using extreme rapid extraction method.
What is the function of CL buffer in DNA extraction?
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
