Why are bananas used in DNA extraction?
Maybe because bananas are annual kind of plant, which means they can survive in all year round in both rainy season and summer season and it's easy to produce unlike to the other plant to our surroundings. On the other hand, bananas have a juicy stem or body, it has a complete structures of genetic information and besides banana can reproduce itself, without mating from the other plant nor from the other species of banana.(asexual reproduction).
DNA is insoluble in isopropanol and therefore, it is ideal for DNA extraction because it allows the DNA to precipitate. The isopropanol is also ice cold because it helps keep the DNA in shape so that it can be readily observed.
To extract DNA from a person you need it in liquid form (i.e. spit) or if it's from a plant you can just grind up the plant in a blender to open up the cells. You need to add detergent and meat tenderizer. Detergent breaks down the lipid bilayer and meat tenderizer breaks down the protein surrounding the DNA. Let it sit for about 15 minutes so that everything can be broken down. Then add… Read More
It is usually done to obtain genes for antibodies.
The reagents used in DNA extraction contains salts that help buffer the respective reagents. Toward the end of DNA extraction, it is necessary to wash away these salts in order to obtain clean DNA for further experiments. Therefore, wash buffer is used for DNA extraction
To study genomic makeup, variations or gene expression, DNA and RNA from cells have to be isolated before any more tests can be conducted. Reasons for isolating genomic DNA include genome sequencing and comparative genomic hybridization (a method for determining gene copy number changes in abnormal cells). Isolating plasmid DNA from bacteria is essential to the processes of bacterial transformation and molecular cloning. Isolating total RNA from a cell is usually used to make cDNA… Read More
link below will help
Because the phosphorus group is more susceptible to change than in a strawberry DNA.
the benefit to extract DNA from banana IS TO PRODUCE NEW AND MORE RAPID POPULATION OF FRUITS
we can use it when we are hukahan
precipitates the DNA out of solution so it can be spooled/hooked and redissolved in TE buffer.
The role of RNase in DNA extraction in terms of purification. For DNA extraction, the sample should be pure. It has to be free from RNase or ribonuclease A.
RNAse destroys the RNA and hence RNAse contamination is a problem in RNA extraction as it breaks down RNA. RNAse enzyme is removed by using RNAse inhibitor or precautions like wearing of gloves, autoclaving tips , using RNAse free water/DEPC treated water is done while performing RTPCR
MgCl2 is used to preserve the integrity of membrane system by counteracting the fixed negative charges of membrane phospholipid. Depending on what you want to extract, it tries to protect the component you are interested in (DNA/RNA/red blood cells, etc) from being lyzed by broken-open lysosome for instance.
liquid nitrogen (-170 C) which it freeze the tissue to become fragile to be a fine powder which increase the surface area of extraction, and the very low temperature prevent DNase activation * the primary function of this step is to lyse the cells
some enzyme has degradation of cell membrane &also degrade protein & lipid of the cell....
cut the cell and remove the dna in cell
used for cell wall lysis
It helps break the nuclear membrane of the cell. Detergent containing the compound SDS ( sodiumdodecyl sulfate) is used to break down and emulsify the fat and proteins that make up a cell membrane.
Mutating a particular DNA sequence into something more interesting. Probing the DNA for known sequences. Digesting the DNA into the little pieces in order to analyze them.
Because, Plant cells are harder to extract DNA from, because it has a Cell Wall. Animal Cells are much easier to obtain.
Strawberries are good to use in a DNA extraction experiment because they have 8 times the amount of DNA than the average cell.
Single molecules of DNA are long and stringy. Each cell of your body contains six feet of DNA, but it's only one-millionth of an inch wide. To fit all of this DNA into your cells, it needs to be packed efficiently. To solve this problem, DNA twists tightly and clumps together inside cells. Even when you extract DNA from cells, it still clumps together, though not as much as it would inside the cell.
increase the chemical reactions
the washing buffers contain ethanol which precipitating DNA molecules and form clumps. DNA is insoluble in alcohol so come together in an alcohol buffer.
I am trying this as I type this. I read on a website what to do: get a DNA source (I plucked fresh grass from outside), blend it in a blender with 1/8 tsp salt and a cup of cold water, strained it, added 2 tbs. detergent, let it sit, and then put some of the solution in a small glass container (I used a 2oz size mini glass ketchup bottle), stir, after that add… Read More
pure water, no.
When extracted from many cells in a sample, using various solvents, the DNA can easily be collected and seen with the naked eye.
phenol is used to denature the proteins.
Plasmid isolation has a step called washing step that carried out in the column in which the plasmid DNA are already bind. There are two wash solution, first one endo wash buffer that wash the traces of bacterial membrane remnants such as LPS. Wash buffer two has ethanol wash off any protein contaminants present on the column. These wash steps ensure the purify of isolated plasmid DNA.
It provides Osmotic Shock to the Blood cells ( leukocytes). By adding sucrose to the solution, it absorbs (osmosis) glucose from the cell, hence cell is shrinked and ruptured. so nucleus is available for extraction.(By Nawaz Naji)
To concentrate or purify the DNA, which is insoluble in isopropanol. Once the solution containing your DNA is placed in isopropanol and centrifuged, the DNA will precipitate to a little pellet at the bottom of your tube. Everything else in your tube is soluble in isopropanol and will remain in liquid form. Pipet the liquid out and now you have just DNA.
The glyco-callyx (sugar-protein) covering of the eukaryotic Cell Coat has much less strength than the mostly cellulose (comprised of strongly bound - bacterially released - glucose) based Cell Wall of the prokaryotic bacteria. Dna extraction is more difficult in prokaryotes due to the tough Cell Wall.
Proteins, RNA, lipids
cloudy, cotton-like, sticky material floating in ethanol
Red blood cells (otherwise known as erythrocytes). They contain no DNA or organelles. They lose them whilst maturing so as to be able to pack more haemoglobin into the cell and therefore be more efficient oxygen carriers.
The only DNA in blood would be the DNA contained in white blood cells as red blood cells have no nucleus and therefore no DNA. Extracting DNA from blood is very easy in a laboratory nowadays. The sample of blood is treated with detergants to break open the cell membrane spilling the contents. Enzymes are now used to break down all the protein, RNA, sugars and fats in the solution. Ethanol (alcohol) is often used… Read More
When the detergent/salt/DNA mixture is agitated, the detergent, along with some inadvertently trapped gas, forms bubbles, and these bubbles may stick to the DNA and the histone proteins. They are not formed by any chemical reaction.
DNA isn't as easily freed from the cells with addition to dish detergent as it would be in wheat germ.
Although it it a bad idea to use something as unregulated as dish washing liquid for DNA isolation, the scientific principle is that proteins denature (or break up) in the presence of a detergent. Denatured proteins can then be separated from the remainder of the cellular contents by centrifugation. This leaves a supernatant containing mainly nucleic acids (DNA and RNA) Dish washing liquid is unregulated because we do not know exactly what is in there… Read More
I'd assume because it has a lot of cells suitable for DNA extraction. I don't really know, we've always used strawberries because if the high amount of seeds in a small space.
Because the cold alcohol reduces the solubility (ability to dissolve) of DNA. It makes the DNA stay intact while the proteins and lipids dissolve or break down.
ethanol change ionic potential of DNA and remove water molecules, which help in precipitation of DNA.
Who cares science sucks!! This would depend on the same person each DNA strand is dedicated to something different such as hair or eye color yet I do not have much experience on the subject and that is just my basic understanding.
DNA should be carried only in plastic tubes because the DNA backbone has a negative charge and the glass tube has a positive charge. The attraction between the two would make the transfer of DNA out of the tube difficult.
When the soap is added, the enzymes in the detergent will break down the lipids in the cell membrane like soap would do to a greasy pan. Also, in the meat tenderizer portion of the lab, the enzymes in the tenderizer will break down the proteins.
No-DNA exists in a microscopic level, you can't obtain it using a blender.
Because it is less dense than the alcohol and make it float