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What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
What is the function of methanol in the wright's stain solution?
to fixed the blood smear
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
How should a smear specimen be fixed and why?
Heat fixed; because it kills the bacteria,firmly affix the smear to the microscope slide, and allow the sample to more readily take up the stain.
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
What is the function of methanol in the wright's stain solution?
to fixed the blood smear
What is protection for elepahnts in heat?
they smear themselves withmud
Reasons why a smear from a gram-positive control showed a gram-negative result?
the smear decolourised more time than it required
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
Why a smear preparation is preferred compare to a wet mount?
bcoz a heat-fix smear have a good contrast than the wet mount
Can you get pregnant if your pap smear test is positive?
yes get pregnant if pep smear test positive
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
What is gram variability?
If after staining, a smear reveals both purple(Gram +) and red cells(Gram -). This is generally an indication that the smear contains gram positive cells.
