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What type of media is used for growing a culture from a throat specimen?
blood and chocolate agar plates as well as in universities laboratory Nutrient agar plates are also provided
The agar used most often for throat cultures is the?
Emb agar
What type of media should be inoculated using zig-zag motion across the surface of the media?
agar slant
What is a Sheep blood agar plate?
A petri dish filled with a nutrient gel containing red blood cells that is used to detect the presence of streptococcal bacteria in a throat culture
What is the name of the streak when you apply specimen to agar plate?
It is called a streak plate, inoculating the agar plate with a zig-zag motion.
When bacteria from a throat swab are streaked on blood agar why is the agar stabbed several times with the loop?
first of all the method of stabbing the agar is no only for throat sample but can be for all specimen except for liquid samples such as urine. the second thing is by stabbing the loop, you can save your time to sterile the loop back. and the other thing is to make sure the loop are no hot anymore..
Does Chromobacterium violaceum pit blood agar?
No it does not pit blood agar I am curently studying Chromobcaterium violaceum and recently grew it on a sheep blood agar it did not pit it. Cv turned the agar a brown color.
Why did you stab the peptone iron agar instead of inoculating only the surface?
Because the peptone iron agar is used to detect ANAEROBIC bacteria. If you stab it deep into the agar you allow the bacteria to grow in the absence of oxygen. If you only inoculated the surface the bacteria wouldn't grow.
A common medium used for growing fastidious bacteria is?
Blood Agar, and trypticase soy agar grows semi fastidious
What is the purpose of stabbing the blood agar?
By stabbing the blood agar, the hemolysis will be easier to see and identify.
How do you make pitri dish gel agar?
Agar is generally made by mixing the powdered form with varying ingredients. The powder is based on seaweed extract for nutrient agar, and nutrient agar is generally a base for most other agars - eg. Horse Blood Agar is nutrient agar with horses blood added; Choc agar has defibrinated (cooked) horses blood added.
Why is it necccessary to place the inoculated molten agar cultures in an iced water bath for their rapid solidification?
to ensure that the cells remain dispersed in an iced water bath.
