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Lactate accumulates because of the lack of available oxygen in the muscles. In anaerobic conditions, the pyruvate produced by glycolysis is reduced to lactate via lactate dehydrogenase (while also oxidizing a single molecule of NADH to regenerate NAD+). NAD+ is a very important molecule and must readily be available in the cytoplasm in order for glycolysis to proceed.

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Q: Why does lactate accumulate in the blood during bursts of very vigorous exercise?
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