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Why does the agar plates has more colonies on after then before?
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What is the difference between himedia ready to use nutrient agar plate and nutrient agar plates prepared by dehydrated media?
Ready to use plates are more expensive because the main difference between the two is the amount of work you have to do. Already made plates and ready to go. Dehydrated you need to make the agar from scratch and pour into empty petri, and wait for it to solidify before you can use them.
What is the function of pound Agar?
LB Broth allows you to create a suspension of bacterial cells from your original colony growing on an agar plate. It provides the necessary nutrients and environment for optimal replication. In suspension, bacterial cells can be plated onto more agar plates, in order to create a streak to cultivate colonies from a single cell, or used to in purification reaction in order to extract the transformed plasmid.
Why are the colonies growing on the top of the agar larger than the colonies growing in the agar?
Well, first of all, the colonies growing on the surface of the agar medium are aerobic . . . that is, they need air - Oxygen - to survive. The anaerobic colonies growing within the agar medium may simply be slower growing or maturing, for some reason. Perhaps that is simply due to their being anaerobic.
What are the uses and advantages of slant agar?
Slant agar is great to use because it allows researchers to consolidate a wide variety of bacteria they wish to store in a very small space such as a test tube rack. It has the similar benefits of TSA plates, but can be kept much easier.
Which technique agar or broth provides more information?
Out of the two, nutrient agar will provide more information on the variety of bacteria that are in an environment. Nutrient agar is a better environment for bacteria and fungi to thrive in.
What are the Advantages for growing bacteria in a broth and on agar plate?
Agar plates gives you a more visual view of the bacteria growth but is limited in the amount of bacteria that can grow on the plate. With broth, you won't be able to see the bacteria colonies but you will be able to grow much more of the bacteria for sampling.
What is the difference between himedia ready to use nutrient agar plate and nutrient agar plates prepared by dehydrated media?
Ready to use plates are more expensive because the main difference between the two is the amount of work you have to do. Already made plates and ready to go. Dehydrated you need to make the agar from scratch and pour into empty petri, and wait for it to solidify before you can use them.
What is the advantage of using a nutrient agar plate over a nutrient agar slant?
Slants are better suited than agar plates, because they can be capped, preventing the agar and the culture from drying out. The cap also prevents airborne contaminants from entering the slant. Also, slants take up less storage space than an agar plate.
What is the function of pound Agar?
LB Broth allows you to create a suspension of bacterial cells from your original colony growing on an agar plate. It provides the necessary nutrients and environment for optimal replication. In suspension, bacterial cells can be plated onto more agar plates, in order to create a streak to cultivate colonies from a single cell, or used to in purification reaction in order to extract the transformed plasmid.
Why are the colonies growing on the top of the agar larger than the colonies growing in the agar?
Well, first of all, the colonies growing on the surface of the agar medium are aerobic . . . that is, they need air - Oxygen - to survive. The anaerobic colonies growing within the agar medium may simply be slower growing or maturing, for some reason. Perhaps that is simply due to their being anaerobic.
Which environment has the most total bacteria either petri dish or liquid agar?
Actually the liquid agar medium is used in the petridishes to grow the colonies of bacteria. The nutritional environment available to the bacteria results in to faster growth of their colonies and also they can be used for the experiments more rapidly
What are the uses of streo microscope?
A stereoscope is used for looking at bacterial colonies on agar plates. Some colonies are very tiny and the stereoscope magnifies them so the technician can perform more detailed testing and observe colony morphology more closely.
What kind of agar should be used to grow bacteria from hands before and after washing for a grade 8 science fair?
blood or nutrient agar would work, but blood agar will most likely grow more bacteria.
Why is it important not to open sterile agar plates?
If you do open one or more of them, you can contaminate them with microbes. These will most likely not be the ones you are trying to culture. It can give you a false negative.
If you strongly suspect a nutrient agar slant culture is contaminated what method would you use to ascertain whether you are right or not?
You have to make subculture from this slant and after incubation you can observe how many types of microorganisms are present in the nutrient agar slant. If you have one colony shape so you have a pure nutrient agar slant but if you have more than one type of colonies so the nutrient agar slant is contaminated.
What does the streak plate method have over the pour plate method?
It is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.
Use of agar slant?
An agar slant is when a test tube is filled with liquid agar and allowed to cool and harden at an angle (slant). Agar is mixed with other nutrients to provide a medium for which bacteria can grow on.
