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The blue flame is hotter then the orange one. Plus the temperature of the flame is not constant in a orange flame but it is more constant in a blue one.
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
It is called a pilot light.
The eternal flame is designed to stay lit indefinitely by using a constant fuel source, such as natural gas or propane, which is continuously supplied and monitored by a system. The flame's design and location also help protect it from wind or other elements that could extinguish it.
The temperature of the flame.
The blue flame is hotter then the orange one. Plus the temperature of the flame is not constant in a orange flame but it is more constant in a blue one.
Pass the loop through a flame 2 or 3 times for about 10 seconds at a time.
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
We use to flame the inoculating loop after inoculation because during inoculation many bacterial cell get attached to loop which can further contaminate the inoculation of other cells so to destroy the previous sticked celled it is necessary to flame burn the loop
when we show the flame in the loop ,for 1 minute its gets brownish colur
The Ner Tamid or Eternal Flame is a representation of the eternal flame that existed in the Great Temple in Jerusalem. In that Temple, there were flames on the sacrificial altar that were always burning. This constant light is a symbol of God's constant presence.
cool flame is used when you want to maintain your liquid at a constant elevated temp.
Aside from the inoculating needle, an inoculation loop, is a simple tool used mainly by microbiologists to retrieve an inoculum from a culture of microorganisms. Its tip is a wire made of platinum or nichrome, the latter being inferior but less expensive. The wire forms a small loop with a diameter of about 5 mm. This loop is handy for taking an inoculum from a liquid by using the phenomenon of surface tension. It is also called a smear loop, inoculation wand or microstreaker, The loop is used to cultivate microbes in Agar jelly and to use the streak maneuver to transfer microbes. The inoculation loop is always sterilized in a flame until it becomes red hot before and after each use. By doing this, the same tool can be reused in different experiments without fear of cross-contamination. After flame sterilization, the loop must be cooled so that the next cells to touch the loop don't instantly die.
http://ask.metafilter.com/25940/How-do-bic-lighters-maintain-such-a-constant-flame
It is called a pilot light.