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Can you use spirit for DNA precipitation?
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
Role of mgcl2 in DNA isolation?
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
Why extraction buffer is added for isolation of DNA?
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
Can you use spirit for DNA precipitation?
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
What is the Role of NaCl in DNA isolation?
NaCl provides Na+ions which form ionic bond with the negatively charged phosphate of DNA,thus neutralizing the effect of negative ,negative repulsion of DNA and helps the DNA molecules to come closer and compact to simplify our process of DNA isolation... BY FARHANA RIYAZ JEZAN UNIVERSITY SAUDI ARABIA.
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
Why heparin can not be used in DNA isolation methods?
heparin may be extrected along with DNA
Why 70 percent alcohol and absolute alcohol used in DNA isolation?
70% ethnol is used for the dna isolation becuse it makes hydrogen bonding with the water molecules and makes DNA hydrophopic so that it pricipitated.
What does DNA isolation used for?
Gene sequencing and gene cloning
Role of mgcl2 in DNA isolation?
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
What is use of ethanol in DNA isolation?
Ethanol is used to precipitate the DNA. I.e. to bring the DNA out of solution. Precipitated DNA is then spun down and re suspended in the appropriate buffer that is suitable for sample storage
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
Why extraction buffer is added for isolation of DNA?
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
How many volumes of isopropanol is to be used for alcohol precipitation of DNA?
0.6 volume
