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Why is hplc a better than uv spectroscopy analasis of aspirin?
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How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
What can affect purity of aspirin?
Firstly place 100 to 150 mg of aspirin into a 125 ml conical flask. Next proceed to mix in 15 ml of 95% ethanol solution and add 2 drops of phenolphthalein indicator. Then use the titration method to mix this solution against a standard solution of sodium hydroxide from a burette. Using the value obtained from the titration calculate the molarity of the aspirin. Then calculate the ratio of the observed molarity of aspirin with its theoretical molarity and finally multiply this ratio with 100 to obtain the percentage purity of the aspirin sample.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
Where can one purchase HPLC detectors?
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
How do you optimise peak separation in HPLC?
we can optimize peak separation by optimizing the HPLC conditions for standard solutions.
