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Nutrient broth contains beef extract which acts as sources of amino acids, peptides, nucleotides, organic acids, vitamins and mineralsthat peptone broth don't contain? :)
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∙ 15y ago
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In Selenite broth why subculture from the top of the broth after incubation?
because it is more motile than other coliforms and can be isolated from the surface if present
If you strongly suspect a nutrient agar slant culture is contaminated what method would you use to ascertain whether you are right or not?
You have to make subculture from this slant and after incubation you can observe how many types of microorganisms are present in the nutrient agar slant. If you have one colony shape so you have a pure nutrient agar slant but if you have more than one type of colonies so the nutrient agar slant is contaminated.
How do you determine if a broth is sterile?
Check for any non-granular sediments if the broth was stationary.Check for turbidity in the broth.Turbidity can be checked by tilting the flask towards light and slowly rotating it while keeping an eye on any difference in light transmittance/color/texture/threads/anything that is different from the rest of the broth!If not sure, autoclave it again!!P.S. Turbidity and sediments can only be visible if there was ample incubation, it will NOT be visible at the moment you make it!!Other advanced way can be in the use of spectroscopy. Take fresh broth as blank and suspected broth as sample and check for optical densities. If the suspect one has a greater turbidity than the fresh one then probably your broth is contaminated.P.S. Right wavelength is the key! It should be set according to the color of your broth!Good luck!
Why is nutrient agar used for bacteria and not for fungi?
The components in the nutrient agar is enriching bacteria than fungi. fungus require additional nutrients which are formulated in fungal media. But in worst case, you can try either media for fungi or bacteria.
Do women have better dexterity than men?
According to scientific studies, women do have better dexterity than men do. This is because women have better sensory-motor coordination than men.
Is Better than bouillon chicken base considered clear broth?
chicken broth is considered clear broth. Better than bouillon will make a fine liquid for any recipe calling for clear broth. Taste it before you add it to your dish. It should be good tasting and you may want to adjust the amount of water per base to your taste.
Why does E-coli grow in nutrient broth better than in thio-glycolate broth?
The purpose of the thio-glycolate broth, which, if memory serves, is a semi-solid agar, is to determine oxygen requirements. E. coli is a facultative anaerobe which means it prefers aerobic conditions but can grow (to a lesser extent) anaerobically. So you will see growth at the top of the thio-glycolate tube mainly where there is oxygen, perhaps some just along the shallow stab line from inoculation. It will not permeate throughout the medium since it prefers aerobic respiration and oxygen is available at the top.
Can you substitute chicken broth for turkey stock?
Yes, you can substitute chicken broth for turkey stock as the two are similar in taste. This would work better than substituting with vegetable or beef stock.
What is the difference between nutrient broth and nutrient agar and can you elaborate more?
When 2%agar is added to nutrient broth ,it is called nutrient agar.Nutrition Broth is liquid media containing beef extract and peptone.Nutrition Agar is solid media containing beef extract, peptone, and agar
What is low sodium broth?
Many brands of beef or chicken broth will market a “standard” or “regular” broth, as well as a low-sodium version. The biggest and most obvious difference between the two options is that the reduced-sodium broth has less sodium in it than the “regular” broth. AustralianBoneBroth
Is brovil considered a broth?
Bovril is considered more of a meat extract than a broth. Bovril can be used to flavor soup.
What can you substitute for whipping cream that is a good nutrient?
the answer would be egg whites it has all the nutrients you need just better than whipping cream
In Selenite broth why subculture from the top of the broth after incubation?
because it is more motile than other coliforms and can be isolated from the surface if present
What nutrient is digested by more enyzmes than any other nutrient?
Protien
Does soup with vegetables take longer to boil than clear broth?
Yes, given that the vegetables have more mass than just plain broth. The denser the objects, the longer it will take to heat.
What is peptone iron agar?
peptone iron agar is used to demonstrate the production of hydrogen sulfide. ıt is more sensitive than lead acetate agar. some organisms have the enzyme to liberate hydrogen sulfide from sulfur containing organic compounds-such as protein-. when H2S is liberated by the enzyme,, the sulfur of the H2S combines with the iron in the PIA medium to form an iron sulfide. The iron sulfide is manifested as a black precipitate in the medium. so you can use in the separation and identification of bacteria. Peptone Iron Agar Approximate Formula* Per Liter Peptone ................................................................... 15.0 g Proteose Peptone ....................................................... 5.0 g Ferric Ammonium Citrate ........................................... 0.5 g Sodium Glycerophosphate ......................................... 1.0 g Sodium Thiosulfate .................................................... 0.08 g Agar ......................................................................... 15.0 g
Is fake butter better than real butter?
No fake butter is not better than real butter because real butter contains natural taste, proteins, nutrient. Where as fake butter consist of chemicals and nothing is real in fake butter.
