as a couterstain
Spirochetes are not considered Gram positive or Gram negative. They tend to stain pink due to the last safrinin dye in the staining process, but its cell wall structure should make it Gram negative.
Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
No. It is a staining on the cell itself.
neither...it is a virus, and gram-staining, a microbiology tool, is not used to help identify or classify a virus
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
Spirochetes are not considered Gram positive or Gram negative. They tend to stain pink due to the last safrinin dye in the staining process, but its cell wall structure should make it Gram negative.
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
A basic dye used in gram staining is crystal violet.
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
No. It is a staining on the cell itself.
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
Gram Staining is a way to separate one large groups of bacteria into two. Crystal violet is used to dye the cells, those that retain the color are grouped as Gram-positive, and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic, making this process useful for detecting infections. A link is provided to permit a quick trip to the Wikipedia article on this topic.
neither...it is a virus, and gram-staining, a microbiology tool, is not used to help identify or classify a virus
You won't be able to positively distinguish gram negative from gram positive organisms. Crystal violet is used first so that gram positive organisms will take up the dye and this color is not affected by safrinin later on. Gram negative organisms will lose the purple coloring during the decolorizer step, therefore need to be counterstained so that you can view the organisms.