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A blast of carbon dioxide is used to check whether a tube or tubes are open. The technician can tell by the degree of backpressure whether one, both, or none of the tubes are open or blocked.

If the tubes are both blocked, no carbon dioxide will get through to the abdominal cavity. If one or both tubes are open, the carbon dioxide will enter the abdominal cavity, and when you sit up, it will cause pressure on the diaphragm (the muscle below the lungs that makes the lungs fill and empty), which causes a stab of Back pain.

Carbon dioxide is used because it is quickly and harmlessly absorbed by the body tissues.

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Q: Why is there pain with carbon dioxide in a tubal ligation?
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