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What would happen if no heat fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
What is the importance of heat fixing the slide before staining?
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
What is Passing a slide sample through a flame is called?
heat fixing
Is it bad to apply heat to a black eye?
yes itz extreamly bad apply cool things such as ice in a towel the coolnes will umb the pain and also help the blood cells react quickly
What would happen if no heat fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
Why must smear on the slide be completely dry before heat fixation?
Slight heating helps in fixing the cells on to the surface of the glass slide
What is the importance of heat fixing the slide before staining?
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
What would happen if no fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
Do animal cells require light?
No but a person requires light to live because it is a heat source.
What is the purpose of heat fixing the smears?
It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without serious distortion. They adhere better to the slide and also take up dye more easily.
What will happen if too much heat is applied while heat fixing a slide?
When too much heat is applied during the heat fixing of a slide with a bacterial cell on it, the cell would explode. The membrane of the cell would rupture.
Upon simple staining procedure you failed to heat fix your E. coli smear and on examination how would you expect this slide to differ from the correctly prepared slides?
heat fixing your slide causes the cells to stick to the slide, without that the cells will be washed right off and there will be nothing left to observe.
What is fixing?
a heat fix is something you put on the slide
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
