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When too much heat is applied during the heat fixing of a slide with a bacterial cell on it, the cell would explode. The membrane of the cell would rupture.
Slight heating helps in fixing the cells on to the surface of the glass slide
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
Heat fixing is done to kill the specimen; adhere the specimen to the slide; and alter the specimen so that they more readily accept stains.
to allow the excess water to dry out before heating. if heated right after, the water would cause the smear to overheat and denature some features in the stain. and those features would no longer be seen
a heat fix is something you put on the slide
When too much heat is applied during the heat fixing of a slide with a bacterial cell on it, the cell would explode. The membrane of the cell would rupture.
heat fixing
I guess so, and that's why you put out the flame after that.
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
The purpose of heat fixing the bacteria to the slide is so during the gram staining procedure the bacteria doesn't wash off. If you didn't heat fix the bacteria to the slide, it would not stay on the slide.
Slight heating helps in fixing the cells on to the surface of the glass slide
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without serious distortion. They adhere better to the slide and also take up dye more easily.
Heat fixing is done to kill the specimen; adhere the specimen to the slide; and alter the specimen so that they more readily accept stains.
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining