Yes,it is an example of non protein enzyme.
You seem confused. RNA polymerase is the enzyme that transcribes DNA into pre mRNA. So, the enzyme would transcribe the messenger RNA for its own protein construction.
THat would be the enzyme DNA Polymerase III which attaches free floating nucleotides to the parent strand. But remember, they can only be attached to a free 3' position!
that the DNA polymerase could be denatured
Replication would be hard pressed to take place. Helicase is the enzyme that splits the double helix and unwinds this helix so that DNA polymerase can do it's job of running the leading and lagging strands of DNA in the replication process.
DNA polymerase can fill the gaps in the DNA that are left by removal of damage bases. DNA polymerase can help cancer cells to tolerate DNA damage.
DNA helicase
You seem confused. RNA polymerase is the enzyme that transcribes DNA into pre mRNA. So, the enzyme would transcribe the messenger RNA for its own protein construction.
THat would be the enzyme DNA Polymerase III which attaches free floating nucleotides to the parent strand. But remember, they can only be attached to a free 3' position!
Transcription would be affected because the binding of the enzyme RNA plymerase to the promoter of the gene is the first step of transcription.
that the DNA polymerase could be denatured
the enzyme helicase unwinds DNA, then DNA rewinds itself back up after translation has finished.
It depends on what type of Enzyme. Enzymes have different optimum pH depending on the environment they work in, for example and enzyme in the stomach of a human would have a pH of about 2 but an enzyme in human saliva has an optimum pH of 5.6.
3'-5' is a characteristic feature of DNA-polymerase I. This activity is meant to repair any misparing mistakes that the enzyme may commit during the synthesis, in which the enzyme would reverse its direction by ONE NUCLEOTIDE and excised the mistakenly added nucleotide, the enzyme acts at the phosphodiester bond at the 5 prime. Whereas the 5'-3' exonuclease activity is an also repair strategy exercised by the DNA polymerase I. However, in this case the polymerase would move in the forwards direction and excise the miss-matched nucleotides at any position regardless with one nucleotide far or so many. This mechanism of repair is well documented in case UV-mutation.
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
Helicase and RNA polymerase separate DNA strands by breaking the hydrogen bonds between complementary bases.Helicase parts the strands of DNA during DNA replication, and RNA polymerase parts them during transcription.The enzyme that separates DNA in called DNA helicases. There are two of them that work away from the origin of replication, creating in "bubble" in the DNA molecule. For eukaryotes, there would be several origins of replication but in prokaryotes, there is only one origin of replication.
it will prevent the transcription of rNA polymerase
There is not really such a thing as a mirror image of DNA in nature. DNA polymerase may be the "molecule" that you are thinking of, it is an enzyme that replicates DNA. When the polymerase makes a new strand of DNA, it uses an existing strand of DNA as a template. The new strand of DNA is not in fact a mirror image of the template strand, but it is the closest thing possible. The new strand is called a complementary strand, not a mirror image.Existing DNA (template for polymerase): ATC TGA CCG GAC TAG GGTNew strand (made by polymerase): TAG ACT GGC CTG ATC CCAAlternatively, by mirror image of DNA you may be thinking of RNA, a ribonucleotide that is made by RNA polymerase. The process is similar to that described above, but the new complementary strand is made out of ribonucleic acid rather than deoxyribonucleic acid.