0
Would methylene blue stain be substituted for safranin as the counter stain in this procedure?
Wiki User
∙ 6y ago
Methylene blue is a stain used in microbiology. The dye attaches to the object, which allows what would normally be fairly see-through to be visible to the microscope.
Wiki User
∙ 9y ago
Wiki User
∙ 13y agoMethylene blue would NOT make an effective counterstain. It stains G-positive cells and G-negative cells in the same manner as the initial crystal violet stain.
Wiki User
∙ 13y agoGram negative cells would be blue and Gram positive would be purple, which would make it difficult to distinguish the two under the microscope.
Wiki User
∙ 12y agoyes
Add your answer:
Earn +20 pts
What color would the G- cell be if not counter stained with safranin?
Purple
Can dyes or other methylene blue be used for direct staining?
Crystal violet, basic fuchsin, and safranin are all dyes which can be used in direct staining because they are cationic which means that they are positively charged. These dyes which are positively charged will react to the bacterial cell wall because the cell wall is negatively charged resulting in a basic stain.
What is secondary and counter stain?
counterstains are selected to be contrasting color so that the target of the primary stain can easily be differentiated on a contrasting background. This makes life easier, when, for example you need to count the number of nuclei in a smear, or number of gram positive bacteria in a mixed population.
Which tool is used to determine the amount of radioactive DNA in a solution?
Geiger Counter
Where do apples rot faster in a fridge or on a counter?
counter
What is the counterstain in a spore stain?
the counter stain is safranin 0.5%
Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
Safranin
What counter stain is used in gram staining?
safranin
What color would the G- cell be if not counter stained with safranin?
Purple
Can dyes or other methylene blue be used for direct staining?
Crystal violet, basic fuchsin, and safranin are all dyes which can be used in direct staining because they are cationic which means that they are positively charged. These dyes which are positively charged will react to the bacterial cell wall because the cell wall is negatively charged resulting in a basic stain.
Which step of a gram stain can be omitted without affecting determination of the reaction?
you can omit the last step - the safranin counterstain
Why is methylene blue is an appropriate dye for staining bacterial cells?
It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.
What is secondary and counter stain?
counterstains are selected to be contrasting color so that the target of the primary stain can easily be differentiated on a contrasting background. This makes life easier, when, for example you need to count the number of nuclei in a smear, or number of gram positive bacteria in a mixed population.
Is teeth whitening an expensive procedure?
Teeth whitening can be done at home with an over the counter kit purchased from a drug store or grocery store, or it can be done professionally be a dentist. The cost will depend on the technique and products used. Over the counter kits are relatively inexpensive, but give you over the counter results.
Can you buy steriods over the counter in Canada?
No,you can't.Methadone is an opioid agonist which can be substituted for opioids such as heroin,to prevent the onset of withdrawal symptoms;it is itself addictive and should only be taken when prescribed to you by your doctor.It is a POM (Prescription Only Medicine)
What is gram stain reaction for Borrelia Burgdoferi?
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
Describe the sequence of events that takes place when an interrupt is raised?
Interrupt Handling 1. Hardware stacks program counter, etc. 2. Hardware loads new program counter from interrupt vector. 3. Assembly language procedure saves registers. 4. Assembly language procedure sets up new stack. 5. C interrupt service runs (typically reads and buffers input). 6. Scheduler decides which process is to run next. 7. C procedure returns to the assembly code. 8. Assembly language procedure starts up new current process.
