Interesting question. I don't think there would be an issue, for the following reason:
Scientists actually deliberately put short sections of RNA made from the wrong strand of DNA in to cells sometimes. It's done because the short section of RNA from the wrong strand of DNA binds to the naturally made mRNA transcript. Ribosomes aren't able to translate double stranded RNA so if you put enough RNA from the wrong strand in you actually stop (or nearly stop) expression of the protein from that gene. From a lab experimental perspective, that's really useful. It's called small interfering RNA and the process overall is known as RNA interference (you can read about both on Wikipedia). I don't think RNA interference as a technique has a problem with cell death (which is generally what happens when cells are unhappy).
I suspect it would also be quite difficult for a cell to make a protein from the wrong strand of DNA, simply because transcription starts with the codon for methionine, which would be missing at the beginning of the transcript (you'd get the code for tyrosine instead). In fact, you'd have to wait till what would normally be a tyrosine codon in your 'right' DNA strand before you got a methionine and hence a 'start' codon.
DNA polymerase
When the replication fork is moving towards DNA double strand in the direction 5'- 3', a "Single-strand Binding Protein" (or SSB) -a dnaB gene product- must be removed in order to allow DNA polymerase to add the following nucleotide.
RNA Polymerase is the enzyme responsible for creating a strand of RNA.
DNA is not made into mRNA, it is transcribed by mRNA. The DNA molecule is split into two strands by the enzyme helicase. One strand is the sense strand and the other is the anti-sense strand. Then mRNA nucleotides pair with their complimentary DNA bases on the antisense strand. The enzyme RNA polymerase causes the mRNA nucleotides to bond with one another, forming a strand of mRNA.
Basically, RNA polymerase's role is very similar to that of DNA polymerase. RNA polymerase is an enzyme that is used during transcription in the nucleus. Similar to DNA polymerase, RNA polymerase codes for the complementary nucleotides to a DNA strand. Instead of thymine though, uracil codes with adenine. This coded mRNA strand then travels from the nucleus to the ribsome where translation occurs - the result is protein made from an amino acid chain. To answer your main question - RNA polyermase adds the complementary nucleotides to the DNA strand using uracil instead of thymine. hope that helps :)
RNA polymerase runs in one direction and is making up a single strand of mRNA. So, the strand not copied in the antiparallel double stranded DNA is called the nonsense strand. ( sense strand is copied )
DNA polymerase
When the replication fork is moving towards DNA double strand in the direction 5'- 3', a "Single-strand Binding Protein" (or SSB) -a dnaB gene product- must be removed in order to allow DNA polymerase to add the following nucleotide.
Polymerase are best know for their role in DNA and RNA replication. The polymerase reads the DNA or RNA strand as a template to synthesize a new strand.
RNA Polymerase
RNA Polymerase is the enzyme responsible for creating a strand of RNA.
DNA is not made into mRNA, it is transcribed by mRNA. The DNA molecule is split into two strands by the enzyme helicase. One strand is the sense strand and the other is the anti-sense strand. Then mRNA nucleotides pair with their complimentary DNA bases on the antisense strand. The enzyme RNA polymerase causes the mRNA nucleotides to bond with one another, forming a strand of mRNA.
Basically, RNA polymerase's role is very similar to that of DNA polymerase. RNA polymerase is an enzyme that is used during transcription in the nucleus. Similar to DNA polymerase, RNA polymerase codes for the complementary nucleotides to a DNA strand. Instead of thymine though, uracil codes with adenine. This coded mRNA strand then travels from the nucleus to the ribsome where translation occurs - the result is protein made from an amino acid chain. To answer your main question - RNA polyermase adds the complementary nucleotides to the DNA strand using uracil instead of thymine. hope that helps :)
A positive strand virus is immediately contagious. A negative strand virus has to create proteins to convert into a positive strand virus. it is not contagious until it becomes a positive strand.
RNA polymerase is the enzyme that makes mRNA from a strand of DNA.
RNA polymerase binds to one of several specificity factors, to form a holoenzyme. In this form, it can recognize and bind to specific regions in the DNA.The -35 region and the -10 region comprise the basic prokaryotic promoter, where the RNA Polymerase binds. The DNA on the template strand between the +1 site and the terminator is transcribed into RNA, which is then translated into a protein. At this stage, the DNA is double-stranded ("closed"). This holoenzyme/wound-DNA structure is referred to as the closed complex.
DNA polymerase is an enzyme which synthetizes complementary DNA strand, according to the template strand. So if you have a single-strand DNA, DNA polymerase can sit on it and synthetize the second strand, by the pairing rules - A pairs with T, G pairs with C.