The total protein test is a rough measure of all the proteins found in the fluid portion of your blood. Specifically it looks at the total amount of two classes of proteins: albumin and globulin.
Proteins are important parts of all cells and tissues. For example, albumin helps prevent fluid from leaking out of blood vessels. Globulins are an important part of your immune system.
How the test is performedBlood is typically drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.
Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm.
Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.
In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.
The blood sample is placed into a machine called a centrifuge, which spins the blood to separate the the liquid part of the blood (the serum) from the cells. The total protein test is done on serum.
How to prepare for the testYour health care provider may tell you to stop taking certain drugs that can affect the test.
Drugs that can increase total protein measurements include anabolic steroids, androgens, corticosteroids, dextran, growth hormone, insulin, phenazopyridine, and progesterone.
Drugs that can decrease total protein measurements include ammonium ions, estrogens, hepatotoxic drugs, and oral contraceptives.
Why the test is performedThis test is often done to diagnose nutritional problems, kidney disease or liver disease. If total protein is abnormal, further tests must be done to identify the specific problem.
Normal ValuesThe normal range is 6.0 to 8.3 gm/dl (grams per deciliter).
Normal values may vary slightly from laboratory to laboratory.
What abnormal results meanHigher-than-normal levels may be due to:
Lower-than-normal levels may be due to:
Additional conditions under which the test may be performed:
Special considerationsTotal protein measurement may be increased during pregnancy.
ReferencesBazari H. Approach to the patient with renal disease. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier; 2007:chap 115.
Klein S. Protein-energy malnutrition. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier; 2007:chap 234.
The total protein test measures the total amount of two classes of proteins found in the fluid portion of your blood: albumin and globulin.
Proteins are important parts of all cells and tissues. For example, albumin helps prevent fluid from leaking out of blood vessels. Globulins are an important part of your immune system.
How the test is performedA blood sample is needed. For information on how this is done, see: Venipuncture
How to prepare for the testYour health care provider may tell you to stop taking certain drugs that can affect the test.
Drugs that can increase total protein measurements include anabolic steroids, androgens, corticosteroids, dextran, growth hormone, insulin, phenazopyridine, and progesterone.
Drugs that can decrease total protein measurements include ammonium ions, estrogens, hepatotoxic drugs, and Birth Control pills.
Why the test is performedThis test is often done to diagnose nutritional problems, kidney disease or liver disease. If total protein is abnormal, further tests must be done to identify the specific problem.
Normal ValuesThe normal range is 6.0 to 8.3 gm/dL (grams per deciliter).
Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.
The examples above show the common measurements for results for these tests. Some laboratories use different measurements or may test different specimens.
What abnormal results meanHigher-than-normal levels may be due to:
Lower-than-normal levels may be due to:
Total protein measurement may be increased during pregnancy.
ReferencesBazari H. Approach to the patient with renal disease. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier; 2007:chap 115.
Klein S. Protein-energy malnutrition. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier; 2007:chap 234.
Tricot G. Multiple myeloma. In: Hoffman R, Benz EJ Jr, Shattil SJ, et al, eds. Hoffman Hematology: Basic Principles and Practice. 5th ed. Philadelphia, Pa: Churchill Livingstone Elsevier; 2008:chap 87.
Reviewed ByReview Date: 05/30/2011
David C. Dugdale, III, MD, Professor of Medicine, Division of General Medicine, Department of Medicine, University of Washington School of Medicine. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.
T protein = Total protein
"Tot Protein" is short for "total protein." The total protein test is a rough measure of all the proteins found in the fluid portion of your blood. Specifically it looks at the total amount of two classes of proteins: albumin and globulin.
Collagen
Total Activity almost always decreases during protein purification.
divide the total grams of protein by 6.25 to find out gm of nitrogen. Calculate the total number of non protein calorie. Divide the total # of NPC by gm of nitrogen will bw the answer.
Total protein, albumin and globulin
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
Yes. Total carbohydrates or total protein or total fat doesn't directly matter in whether one loses or gains weight- it's simply the total number of calories consumed. If you consume too many calories from any source, carbohydrates, protein, or fat, you'll simply store it.
6.4 what?
Total protein
12,5 % from protein
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