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Why you flame the loop when you have finished streaking your plate?
Wiki User
∙ 13y ago
It is important to red hot the loop before streaking so that any microbial cell or if present on loop will burn and it will prevent your culture from contamination. Flaming should be done in each and every time befor you streak even for a single culture and for single plate.
Wiki User
∙ 15y ago
Wiki User
∙ 11y agoTo kill the remaining bacteria on the loop.
Wiki User
∙ 13y agootherwise contamination will occur on the laminar air hood table
Wiki User
∙ 6y agoYou will then be able to produce another streak that has fewer bacteria. The whole idea is to finally have only a few bacterial colonies.
Wiki User
∙ 13y agoTo get isolated colonies.
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Why does the streaking method you used to inoculate you plates result in isolated colonies?
In the streak plate technique, a loop is used to streak the mixed sample many times over the surface of a solid culture medium in a Petri plate. Theoretically, the process of streaking the loop repeatedly over the agar surface causes the bacteria to fall off the loop one by one and ultimately to be distributed over the agar surface, where each cell develops into a colony.
What is streak dilution plate technique?
"dilute" is not really the right word for it but... A proper streaking method (4 streaks total recommended but some hospital policy prefer 3) can separate and isolate the clinically significant bacteria (the one who makes the patient sick). With each streak, the used metal loop must be incinerated or a used plastic loop should be thrown away AND the streaking lines should have space to each other(except the 1st streak) to ensure the none of the flora are being isolated or else getting the correct results will be difficult.
What is the criteria for a good quality streak plate?
In scientific circles, the streak plate method is considered to be a rapid qualitative isolation method. To be effective, one must reduce the number of organisms in the inoculums by spreading a loop of culture over an agar plate. This ensures that individual cells are properly separated on the surface for the purpose of differentiating various species. The method is as follows: Using a sterile loop, microbes are initially transferred to the plate with one swipe. On the subsequent swipes, the loop is heated in the flame of a Bunsen burner to lessen the population of microbes being transmitted. Streak patterns are also done in via T-streak or by applying the loop to four quadrants of the plate.
When is the loop preferable for transferring bacteria?
Subculturing refers to transferring microorganisms from one medium to another. For example, bacteria growing in broth may be transferred to an agar plate.Wire loops are used to transfer microorganisms from liquid media to liquid or solid media.Needles are used for transferring microorganisms to deep tubes.
How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample from an agar plate?
The plate cover is raised and held diagonally to protect the plate surface from any contamination in the air.
Why flaming the loop when streaking for isolation?
To sterilize it.
Why does the streaking method you used to inoculate you plates result in isolated colonies?
In the streak plate technique, a loop is used to streak the mixed sample many times over the surface of a solid culture medium in a Petri plate. Theoretically, the process of streaking the loop repeatedly over the agar surface causes the bacteria to fall off the loop one by one and ultimately to be distributed over the agar surface, where each cell develops into a colony.
What is streak dilution plate technique?
"dilute" is not really the right word for it but... A proper streaking method (4 streaks total recommended but some hospital policy prefer 3) can separate and isolate the clinically significant bacteria (the one who makes the patient sick). With each streak, the used metal loop must be incinerated or a used plastic loop should be thrown away AND the streaking lines should have space to each other(except the 1st streak) to ensure the none of the flora are being isolated or else getting the correct results will be difficult.
What are the steps in properly streaking a plate?
When we have to isolate a specific microbial species from their mix culture or to grow any microbe on solid surface for their further studies then they can be grow on a culture medium containing a gel like substance known as agar which produce disticnt microbial colonies when inoculate in a petri dish containing the growth medium. The way by which the inoculation of microbial sample done is called a streak plate method in which the microbial sample is streak with the help of inoculating loop on the agar plate firmly, in the way so that the cell can be isolated. There are two more method to incoulate the microbial sample that are: pour plate and spread plate techniques.
Why the loop must be flamed before streaking a new group of lines?
the inoculation loop must be flamed before streaking a new group of line to avoid any type of contamination. This is said to be one type of sterilization(dry heat sterilization) process called incineration.
What is meant by streak dilution technique?
The streak plate technique is a method of diluting bacteria down suficiently so that the will grow as single colonies. The technique varies from individual to individual so much so that you can identify a researcher's plates much like their handwritting! The technique is somewhat more standardised in hospital labs and a printed out sheet is placed below the plate for the operative to follow as a guide. The technique is usually taught like this; 1) Flame your loop and aseptically take 1 loopful of culture and place it a 12 o'clock on your plate draw a straight line 5cm across the plate ending around 2.30o'clock. 2) Lift the loop and draw two more lines parallel the first about 0.5 cm distance below the first. 3) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines over lapping the first set. (your end at 5o'clock) 4) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the second set. (you end at 6.30o'clock) 5)Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the third set. (your end at 8o'clock) 6) Flame your loop this time instead of a set of lines start by overlapping the fourth set of lines and then draw a scribble into the middle of your plate using as much of the unused agar as possible. The technique is sort of a dilution becasue each time you flame your loop it is sterilised, when you then draw out some of the bacteria from your last set of lines and spread them over a much greater area.
What is the criteria for a good quality streak plate?
In scientific circles, the streak plate method is considered to be a rapid qualitative isolation method. To be effective, one must reduce the number of organisms in the inoculums by spreading a loop of culture over an agar plate. This ensures that individual cells are properly separated on the surface for the purpose of differentiating various species. The method is as follows: Using a sterile loop, microbes are initially transferred to the plate with one swipe. On the subsequent swipes, the loop is heated in the flame of a Bunsen burner to lessen the population of microbes being transmitted. Streak patterns are also done in via T-streak or by applying the loop to four quadrants of the plate.
How can a contamined wire loop be cleaned?
insert the loop into the burner flame until all of the salt has been burned off
When is the loop preferable for transferring bacteria?
Subculturing refers to transferring microorganisms from one medium to another. For example, bacteria growing in broth may be transferred to an agar plate.Wire loops are used to transfer microorganisms from liquid media to liquid or solid media.Needles are used for transferring microorganisms to deep tubes.
How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample from an agar plate?
The plate cover is raised and held diagonally to protect the plate surface from any contamination in the air.
Why must pay attention to how flame loop?
because it can burn you or the microorganism will not be killed
What is the purpose of flaming the loop after use?
I'm assuming you mean an "innoculating loop" in microbiology. You flame the loop to kill the microoganisms on the loop before using it again to prevent mixing different bacterial colonies and contaminating them.
