enzyme is nothing but the protein which is formed from DNA by translation through transcription of a particular region of the DNA . so obviously the DNA is larger than the enzyme in molecular size.
There is just one enzyme used in the ELISA reaction. This enzyme is linked to the secondary antibody. Commonly used ELISA enzymes are:Alkaline phosphataseHorseradish peroxidase
spliceosomes
Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including obtaining a "map" and cloning DNA. Single digests consitute DNA being treated with one restriction endonuclease, whereas double digests contain 2 enzymes. At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...). If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.
gcgtagg
enzyme linked immunosorbant assay
A restriction enzyme is a protein that cuts DNA at specific sequences, allowing scientists to manipulate and study DNA molecules in molecular biology experiments.
What an enzyme does is based on its shape, therefore you would have to change it on a molecular level in order to alter its job.
Hydrolases - Hydrolysis of a substrate - digestive enzyme isomerases - change of the molecular form of the substrate - famerase
simple enzyme molecular structure
There is a direct relationship; as the enzyme concentration increases, the rate of reaction increases.
Beadle and Tatum concluded that genes control the synthesis of enzymes, proposing the "one gene-one enzyme" hypothesis. This research laid the foundation for the understanding of how genes encode proteins and paved the way for the field of molecular genetics.
Enzymes are typically larger in molecular size than DNA. Enzymes are made up of complex protein structures, which can be thousands of amino acids long, while DNA molecules are composed of nucleotides and are generally shorter in length compared to most enzymes.
One gene controls the synthesis of one enzyme.
Such an enzyme is called a restriction endonuclease
Tobin can conclude that the reaction rate is directly proportional to the enzyme concentration when excess substrate is present. This is because at higher enzyme concentrations, all substrate molecules are already bound to enzyme active sites, leading to a maximal reaction rate even with excess substrate.
The restriction enzyme GGATCC is significant in molecular biology research because it recognizes and cuts DNA at a specific sequence, allowing scientists to manipulate and study DNA molecules. This enzyme is commonly used in genetic engineering techniques such as gene cloning and DNA fingerprinting.
Its molecular shape is altered so the substrate cannot fit to its active site