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What else can I help you with?
How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the smae restriction enzyme use to cut out the hyman gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.
Where do they cut the DNA strand?
They direct a specific Restriction Enzyme to cut the Dna Exactly where required.
What must researchers know before they begin the process of gentic engineering?
you need to know which restriction enzyme to use. also, who is the doner and the plasmid.
Can someone help me with biology home work?
The complementary overhangs of restriction enzymes form a 'scar' between the Promoter and Chromoprotein. This scar cannot be digested by any of the four restriction enzymes used to create BioBricks. This allows for iterative building of more complex BioBricks. Why can the scar not be cut, despite retaining the overhang sequence? Select one: a. Because the scar is not a palindrome. b. Because the scar has a distorted double helix that cannot be recognized by the restriction enzyme. c. Because restriction enzymes only cut at one sequence of DNA. d. Because restriction enzymes recognize additional DNA sequence beyond the overhang region
How do you isolate the gene in genetic engineering?
You isolate the gene in genetic engineering by first locating the gene you wish to be isolated. Then you use a restrictive enzyme to isolate it, and lastly take the gene out
What is The role of buffer in restriction digestion reactions?
I believe that it is used to stabilize the pH levels so the enzyme activity isn't effected.
Restriction enzyme digestion?
Yes?
What are the common challenges faced when dealing with restriction mapping problems?
Common challenges faced when dealing with restriction mapping problems include difficulties in accurately determining the order and distances between restriction sites, resolving overlapping fragments, and interpreting complex patterns of restriction enzyme digestion. Additionally, issues such as incomplete digestion, DNA degradation, and variations in enzyme activity can complicate the mapping process.
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
How do you inactivate MspI restriction enzyme?
it can't be heated to inactivation,instead you can just try protease K treatment
What type of enzyme is used to fragment dna?
Restriction enzymes are used to fragment DNA by cutting it at specific recognition sites. These enzymes are naturally found in bacteria as a defense mechanism against foreign DNA, and are commonly used in molecular biology techniques like restriction enzyme digestion.
Which restriction enzyme did you use to cut the DNA?
The restriction enzyme used to cut the DNA was EcoRI.
Hi does any one know if I want to put some restriction enzyme close to cDNA should I put some nucleotide befor this restriction enzyme?
Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations
What is the role of BSA in restriction enzyme activity?
BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization.
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
What is the difference between a restriction enzyme and an endonuclease?
A restriction enzyme is a type of endonuclease. Endonucleases are enzymes that cut DNA at specific sequences, while restriction enzymes specifically cut DNA at recognition sites called restriction sites.
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
