0
The M13 phage was sequenced using traditional Sanger sequencing methods, which rely on chain-termination technology. This involved breaking down the phage's genome into smaller fragments, sequencing these fragments, and then assembling the sequences to reconstruct the complete genome. This method allowed scientists to determine the linear sequence of the DNA bases in the M13 phage genome.
What else can I help you with?
Why bacillus anthracis susceptible to gamma phage?
Bacillus anthracis is susceptible to gamma phage because the phage has evolved to target and infect specific receptors on the surface of the bacterium. This interaction enables the gamma phage to inject its genetic material into the bacterium, hijacking its machinery to produce more phage particles and ultimately leading to the destruction of the bacterial cell.
When a phage attaches to a bacterium what does the phage inject and what stays attached to the cell?
When a phage attaches to a bacterium, it injects its genetic material (DNA or RNA) into the cell. The phage capsid (outer protein coat) typically stays attached to the cell surface during this process.
What is the difference between a virulent phage and a temperate phage?
In virology, temperate refers to the life cycle some phages are able to perform. Atemperate phage can integrate its genome into its host bacterium's chromosome, becoming a lysogen known as a prophage. A temperate phage is also able to undergo lytic life cycles, where the prophage is expressed, replicates the phage genome and produces phage progeny and the progeny phage leave the bacterium. The virulent phages have only lytic lifecycles and thus infection results in the host cell's death (due to lytic cell destruction-the phage replicates itself and then bursts the cell, releasing many copies).
What phage in the reproductive cycle kills the bacterial host cell?
In the lytic cycle, a phage kills the bacterial host cell by causing it to burst (lysis) to release new phage particles. The phage replicates inside the host cell until it reaches a critical point, then the host cell is ruptured to release the new phages to infect other host cells.
What radioactive isotope did hershey and chase use to tag phage DNA?
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
What is the function of bacteriophage?
The function of bacteria is to reproduce. Bacteria is a large domain of prokaryotic microorganisms. They are also the cause of human and animal diseases. Some bacteria, like those in the intestines are friendly and needed for digestion.
A virus that reproduces in a bacterium is called?
phage
What is another term for a lysogenic phage?
temperate phage
How many stars are in m13?
There are estimated to be about 1 million stars in the globular cluster Messier 13 (M13) located in the constellation Hercules.
When phage attaches to a bacteria the phage injects what?
DNA single
The substance a phage leaves outside its host cell?
The substance a phage leaves outside its host cell is typically referred to as the "phage progeny" or "phage particles." These particles can include newly synthesized phage DNA and proteins, as well as the phage capsid that encapsulates the genetic material. This material can go on to infect other host cells and continue the phage replication cycle.
What is the need for phage titration?
Phage titration is needed to determine the concentration or titer of phages in a sample, which is crucial for ensuring the effectiveness of phage-based therapies, determining the optimal phage concentration for experiments, and monitoring phage growth dynamics in culture. Titration allows researchers to quantify the number of active phages present in a sample, providing valuable information for various applications in phage research and biotechnology.
What is temperate phage superinfection immunity?
c. Repression of the phage genome - A phage coded protein, called a repressor, is made which binds to a particular site on the phage DNA, called the operator, and shuts off transcription of most phage genes EXCEPT the repressor gene. The result is a stable repressed phage genome which is integrated into the host chromosome. Each temperate phage will only repress its own DNA and not that from other phage, so that repression is very specific (immunity to superinfection with the same phage).Reference: http://pathmicro.med.sc.edu/mayer/phage.htm
Is Phage the Untouchable banned in any formats?
Yes, Phage the Untouchable is banned in the Commander format.
Why bacillus anthracis susceptible to gamma phage?
Bacillus anthracis is susceptible to gamma phage because the phage has evolved to target and infect specific receptors on the surface of the bacterium. This interaction enables the gamma phage to inject its genetic material into the bacterium, hijacking its machinery to produce more phage particles and ultimately leading to the destruction of the bacterial cell.
When a phage attaches to a bacterium what does the phage inject and what stays attached to the cell?
When a phage attaches to a bacterium, it injects its genetic material (DNA or RNA) into the cell. The phage capsid (outer protein coat) typically stays attached to the cell surface during this process.
What does a phage inject when it attaches to a bacterium?
A phage injects its genetic material (DNA or RNA) into the bacterium when it attaches to it. This genetic material then hijacks the bacterium's machinery to replicate itself, eventually leading to the destruction of the bacterium.
