The complementary sequence of a DNA strand is written with the beginning letters of the bases: adenine (A), cytosine (C), guanine (G), and thymine (T). You would replace each letter with its complementary nucleotide.
Replace:
A for T
T for A
C for G
G for C
If TACGTT is read 5'-TACGTT-3' then the complimentary strand will read 5'-AACGTA-3'. Since the template strand is traditionally written in the 5' to 3' direction then the complimentary strand, written in the same manner, would be AACGTA not ATGCAA. The four bases, adenine(A), thyamine(T), cytosin(C), and guanine(G) bond together in pairs. A - T, and C - G. They do not pair with any other base unless in the case of RNA, when thyamine is replaced with uracil.
It would be T-A-A-G-C-C
The reaction in the test tube generates a single-stranded complementary DNA molecule when complementary DNA is made for reading DNA. This process uses the enzyme reverse transcriptase to synthesize a DNA strand from an RNA template, allowing for the genetic information encoded in the RNA to be read and manipulated.
The complementary DNA sequence to CTA is GAT. In DNA, adenine (A) pairs with thymine (T) and cytosine (C) pairs with guanine (G). So, for every C in one strand, there should be a G in the complementary strand, and for every T in the original strand, there should be an A in the complementary strand.
If cga ct were used as a template strand for complementary DNA synthesis, the complementary DNA produced would be gct ga. This is because each nucleotide pairs with its complementary base: cytosine (c) pairs with guanine (g), guanine (g) pairs with cytosine (c), adenine (a) pairs with thymine (t), and thymine (t) pairs with adenine (a). Therefore, the complementary sequence would read from 5' to 3' as gct ga.
If TACGTT is read 5'-TACGTT-3' then the complimentary strand will read 5'-AACGTA-3'. Since the template strand is traditionally written in the 5' to 3' direction then the complimentary strand, written in the same manner, would be AACGTA not ATGCAA. The four bases, adenine(A), thyamine(T), cytosin(C), and guanine(G) bond together in pairs. A - T, and C - G. They do not pair with any other base unless in the case of RNA, when thyamine is replaced with uracil.
It would be T-A-A-G-C-C
The complementary strand of DNA for the sequence AGTT would be TCAA. In DNA, adenine pairs with thymine and guanine pairs with cytosine. So the complementary base for A is T, G is C, T is A, and T is A.
When reading a DNA sequencing gel from bottom to top, you are reading the sequence of the complementary non-coding strand of DNA. This is because the gel displays the sequence of bands corresponding to the bases in the DNA template strand, which is the non-coding strand.
DNA strands are said to be complementary because they both match up with eachother; A with T and C with G. So if you have the strand ATGGCTA the complementary strand (the other half of the double helix) would read TACCGAT. So if you know one side of the strand then you can describe the whole.
The reaction in the test tube generates a single-stranded complementary DNA molecule when complementary DNA is made for reading DNA. This process uses the enzyme reverse transcriptase to synthesize a DNA strand from an RNA template, allowing for the genetic information encoded in the RNA to be read and manipulated.
The complementary RNA sequence that would pair with the DNA sequence TACTGCA is AUGACGU. This is because in RNA, uracil (U) is used instead of thymine (T) to pair with adenine (A), cytosine (C) pairs with guanine (G), and vice versa.
The key feature that allows DNA to be copied is its double-stranded helical structure, where the two strands are complementary to each other. During replication, enzymes called DNA polymerases read the existing strand to create a new complementary strand by matching bases (A with T, and C with G). This process ensures that each new DNA molecule contains the same genetic information as the original one.
The complementary DNA sequence of ATCGA is TAGCT. In DNA, adenine pairs with thymine (A-T) and cytosine pairs with guanine (C-G), so to find the complementary sequence we switch A to T, T to A, C to G, and G to C.
The complementary DNA sequence to CTA is GAT. In DNA, adenine (A) pairs with thymine (T) and cytosine (C) pairs with guanine (G). So, for every C in one strand, there should be a G in the complementary strand, and for every T in the original strand, there should be an A in the complementary strand.
Transcription is unidirectional because you are copying only ONE side of the DNA. Remember that DNA is a double stranded helical structure. One strand of DNA is complementary to the other strand.
If cga ct were used as a template strand for complementary DNA synthesis, the complementary DNA produced would be gct ga. This is because each nucleotide pairs with its complementary base: cytosine (c) pairs with guanine (g), guanine (g) pairs with cytosine (c), adenine (a) pairs with thymine (t), and thymine (t) pairs with adenine (a). Therefore, the complementary sequence would read from 5' to 3' as gct ga.