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monoclonal antibodies lies on the principal of producing antibodies and monocloaal means the specificity and avidity for antigen i.i they recognize a single epitope.
firstly the mouse is innoculated with appropriate antigen and spleen cells are isolated from it.
then the myeloma cells grown in vitro and spleen cells which are isolated are fused in the polyethyleneglycol (PEG) and cultured in HAT medium.
the following result comes;
1. spleen cells die in vitro
2. spleen-myeloma hybrid cells survive
3. unfused myeloma cells die in HAT medium
surviving hybrid cells are clonned and used further.
What else can I help you with?
Which monoclonal antibody is marketed under the name Xolair?
Xolair is marketed as omalizumab. It is a monoclonal antibody used to treat allergic conditions such as asthma and chronic idiopathic urticaria by targeting and neutralizing immunoglobulin E (IgE).
How is find the gene sequence of an monoclonal antibody found?
find the protein sequence in protein sequence data base.....then from the protein sequence u can find the antibody gene sequence...... u can also go for nucleotide sequencing....which will directly help u in getting the sequence.....u can check this in bioinformatics data base
What is the valueof monoclonal antibody technology?
monocial antibody technology has become an important tool in biochemistry, molecular biology and medicine.Monoclonal antibodies have monovalent affinity, in that they bind to the same epitope.
Can monoclonal antibodies be specific for multiple antigenic determinants?
Yes, monoclonal antibodies can be specific for multiple antigenic determinants if those determinants share similar structural features that allow the antibody to bind to them. This property is known as cross-reactivity. However, some monoclonal antibodies are designed to target a single specific antigenic determinant to ensure high specificity in diagnostic or therapeutic applications.
What is the value of monoclonal antibody technology?
Monoclonal antibodies can be used to protect against disease, help diagnose a wide variety of illnesses and detect the presence of drugs or viral and bacterial products. It is these specific traits that make it so valuable.
What does MAB mean?
Monoclonal antibody
What is an agonistic monoclonal antibody?
An agonisitic monoclonal antibody is an immunological term for a monoclonal antibody which attempts to boost the immune system in order to fight infection or cancer.
What is the medical definition of a monoclonal antibody?
A monoclonal antibody is a highly specific antibody that has been formed in a laboratory setting. It is made up of clones from a hybrid cell made from a fusion of B-cells with a tumor cell.
What was the name of the first FDA approved Monoclonal antibody for the treatment of rheumatoid arthritis?
Humira (generic name adalimumab) Is The First Human Monoclonal Antibody Approved For Rheumatoid Arthritis.
How and how often is monoclonal antibody therapy for breast cancer given?
Monoclonal antibody therapy is administered through injections. How often it should be perfomed varies depending on what your doctor recommends.
How valuable is monoclonal antibody production?
Monoclonal antibody production can be seen as very valuable as it creates an insight into diagnosing in vitro anomalies. Monoclonal antibodies are derived from cloned cells and allow people to study in depth anomalies that occur in humans and in reproduction.
How monoclonal antibody are produced?
by the help of hybridoma technology. single type of B cell which is sensitized with single epitope of antigen fysed with a myeloma cell. then that fused cell produce single type of antibody....that is monoclonal antibody.....
What does the medical abbreviation MOAB mean?
MoAb stands for "monoclonal antibody"
Why polyclonal antibody solution is able to precipitate its antigen protein whereas monlclonal antibody solution has hard time to do so?
Polyclonal antibody recognizes several epitopes on the target protein while monoclonal antibody recognizes only single epitope. Sometimes, monoclonal antibodies are not able to precipitate the antigen because the epitope might need to be exposed on the surface of the antigen to be recognized by the antibody. Since the epitope might be hidden and it's a single epitope that is recognized by the monoclonal antibody, the propability of the antibody to reconize the epitope is lower compared with the polyclonal antibody that recognizes several epitopes on the target protein.
Which monoclonal antibody is marketed under the name Xolair?
Xolair is marketed as omalizumab. It is a monoclonal antibody used to treat allergic conditions such as asthma and chronic idiopathic urticaria by targeting and neutralizing immunoglobulin E (IgE).
Why polyclonal antibody tend to have cross-reaction compare to monoclonal antibody?
Polyclonal antibody recognizes several epitopes on the target protein while monoclonal antibody recognizes only single epitope, hence monoclonal antibodies are more specific than polyclonal antibodies. However, sometimes MAbs are not able to precipitate the antigen because the epitope might need to be exposed on the surface of the antigen to be recognized by the antibody. Since some of the epitope might be hidden and it's a single epitope that is recognized by the monoclonal antibody, the propability of the antibody to reconize the epitope is lower compared with the polyclonal antibody that recognizes several epitopes on the target protein this is the reason for the tendency of polyclonal antibodies to have cross-reaction as compared to MAbs. by Victor S Gruezo Jr
What is the Genetic engineering technique used for creating monoclonal antibody?
recombinant DNA technology
