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Which of the objectives comes closest to a specimen and is most likely to break a slide if proper precautions are not taken?
The oil immersion objective comes closest to the specimen and is most likely to break a slide if proper precautions are not taken. Oil immersion objectives require the addition of immersion oil between the objective lens and the slide to improve resolution, and without the correct amount of oil or if the slide is not handled carefully, it can lead to damage or breakage of the slide.
Why should you never turn the coarse objective knob downward?
Turning the coarse objective knob downward can cause the objective lens to come in contact with the slide, potentially damaging both the lens and the slide. It is best to always start with the coarse knob in the upward position to prevent any accidental collisions.
Why must the distance from slide to objective lens increase rather than decrease when coarse focusing with the scannin and low power objectives?
Increasing the distance between the slide and objective lens when coarse focusing with scanning and low power objectives allows for a larger range of movement without bringing the lens and slide into contact, thus reducing the risk of damaging the lens or the slide. It also prevents too much light from entering the lens at once, which can result in a blurry or washed-out image.
When you moved the slides in different directions what changes in the image of the specimen under focus did you notice?
You should notice that the image in the microscope moves in the opposite direction to the movement of the slide. For example, if the specimen slide is moved top to bottom, the image seen moves from bottom to top. This can be very confusing.
Why should you only use the fine adjustment when the high power objective is in position?
Because if you use the coarse adjustment under high power, there is a greater risk that you will crack the slide on the microscope and damage it. The fine adjustment only moves the objective lense small amounts and very slowly, so there is no chance that you will damage the slide.
What is the space between the objective and the slide on a microscope?
The space between the objective lens and the slide on a microscope is called the working distance. It is important because it allows for proper focusing and manipulation of the specimen without touching the lens. The working distance varies depending on the magnification and type of objective lens being used.
Why can't the objective lenses touch the slide?
The objective lenses should not touch the slide because doing so can lead to scratches on the lens or slide, affecting the quality of the image. Additionally, touching the slide with the objective lens can potentially damage the specimen being viewed. Maintaining a small distance between the lens and the slide ensures proper focus and clarity in the image.
Is the note pane located above the slide pane and is an area where you can type notes and additional information?
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Which of the objectives comes closest to a specimen and is most likely to break a slide if proper precautions are not take?
The objective lens on a microscope that comes closest to the specimen and is most likely to break a slide if proper precautions are not taken is the high-power objective. High-power objectives are designed for high magnification, typically in the range of 40x to 100x, and they have a very short working distance. The working distance is the distance between the objective lens and the specimen. Because the high-power objective has such a short working distance, it means that it needs to be very close to the specimen being observed. If the slide or coverslip is too thick or if there's an uneven or raised area on the slide, there's a risk that the objective lens may come into contact with the slide, potentially causing damage to both the lens and the slide. This is why it's crucial to handle slides carefully and ensure they are prepared with the appropriate thickness and flatness to avoid damaging the high-power objective and obtaining clear images during microscopy.
When the microscope is changed from low power to high power the distance between the objective lens and the cover slip of the slide will?
Decrease.
Which part of the microscope is used to bring the image of an object on the slide into focus when under the high power objective lens?
The fine adjustment knob is used to bring the image of an object on the slide into focus when under the high power objective lens. This knob allows for precise focusing by making small adjustments to the distance between the objective lens and the specimen on the slide.
Why should you look to the slide before you lower the objective of your microscope?
so the objective lens will not touch the slide.
Why is it not safe to remove slides from the microscope when 100x object is in place?
Removing slides with a 100x objective in place can damage the objective lens or the slide, as the lens is very close to the slide. Additionally, there is a risk of dropping or mishandling the slide when the objective is at 100x, causing potential damage to both the slide and the objective lens. It is safer to switch to a lower magnification objective before removing the slide.
How do you calibrate the objective lens on a light microscope?
To calibrate the objective lens on a light microscope, use a stage micrometer slide with a known scale (e.g. 0.01 mm). Focus on the markings of the stage micrometer slide and measure the distance they cover in the field of view. Use this measurement to calculate the calibration factor for that specific objective lens.
Once focus is achieved at one magnification a higher power objective lens can be rotated into positon without fear of striking the slide true or false?
True. Once focus is achieved with a lower magnification objective lens, a higher power objective lens can be rotated into position without fear of striking the slide because the working distance between the lens and the slide increases with higher magnification lenses.
When observing a slide should the specimen be on the top or bottom surface of the microscope?
The specimen should be placed on the top surface of the microscope slide. This allows the light to pass through the specimen from below and be magnified by the lenses in the microscope to form an image for observation.
Why should you always have to look at a microscope from side when moving an objective?
Looking at a microscope from the side when moving an objective helps prevent accidentally crashing the objective lens into the slide, which can damage both the lens and the slide. It also allows you to have a better view of the distance between the objective lens and the slide, ensuring smooth and precise movement without causing any harm.
