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What reaction does the enzyme involved in Mannitol-salt agar test mediate?

Mannitol salt agar is selective for gram positive bacteria, and differential for mannitol fermenters. Phenol red is the indicator containing the enzyme mannitol.


Does Saccharomyces cerevisiae ferment mannitol?

Mannitol salt agar inoculated with Micrococcus luteusshowing no fermentation of mannitol (pink medium). The colonies show a yellow pigment which is characteristic of M. luteus.


What is the principle of mannitol fermentation?

Mannitol fermentation have high levels of NaCl (7.5%) which inhibit most organism other than staphylococci aureus, contains D-mannitol, pH indicator called 'phenol red' which detect the acid production in mannitol . It also differentiate S. aureus and S. epidermidids .


Does bacillus subtilis ferment acid from mannitol salt agar?

Bacillus subtilis typically does not ferment acid from mannitol salt agar as it lacks the enzymes needed for fermentation. Mannitol salt agar is mostly used to differentiate Staphylococcus species, which are capable of fermenting mannitol.


What color indicate positive mannitol test?

Yellow, which means bacteria is able to produce acids from mannitol fermentation


What causes a color change in Mannitol salt agar?

A color change in Mannitol salt agar is typically caused by the fermentation of mannitol by bacterial species that can utilize it as a carbon source. This fermentation process results in the production of acids, causing the pH to drop and leading to a color change in the pH indicator present in the agar (usually phenol red) from red to yellow.


Does antigens cause fermentation?

no it does not cause fermentation. What do you mean by an Antigen in the context of fermentation? Zymase is the enzyme used in fermentation, when this enzyme used as an Antigen to immumize an animal, it do produce antibody against it :)


What is the commercial way for producing enzymes?

Large fermentation vessels are used for commercial enzyme production.


Why is mannitol salt considered to be a differential media?

Mannitol salt agar is used for the isolation of staphylococci which is found normally on skin (S. aureus). The selectivity is obtained by the high salt concentration that inhibits growth of many groups of bacteria.


Why is only mannitol used in ashby's mannitol broth?

MANNITOL IS CARBON SOURCE & AZOTOBACTER can fix nitrogen non- symbiotically


How enzyme concentration effect the rate of fermentation?

Increasing enzyme concentration typically increases the rate of fermentation by providing more enzymes to catalyze reactions. This results in faster conversion of substrates into products, speeding up the fermentation process. Beyond a certain point, further increases in enzyme concentration may not have a significant effect on the rate of fermentation due to substrate limitations or other factors.


Why is it important to know an enzyme concentration on fermentation?

it speeds it