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it is a series of palindromic base pairs that act as a place where DNA can be cut. In Biology, we can take advantage of this by splicing various genes into and out of bacteria to determine their effects.

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What is a DNA restriction site?

The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.


What is the restriction site of the restriction enzyme TaqI?

TaqI's restriction site is:TCGAAGCT


Can Restriction Enzymes be used on all DNA?

Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis


What determines how DNA will be cut by a restriction enzyme?

Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut


Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?

The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.


What is a palindrome as it relates to a restriction enzyme site?

A palindrome in the context of a restriction enzyme site refers to a sequence of DNA that reads the same forward and backward. Many restriction enzymes recognize and cut DNA at palindromic sequences. This characteristic allows the enzyme to bind symmetrically to both strands of DNA.


What is HinF1 site in DNA sample?

HinF1 site is a specific sequence of base pairs (5'-GANTC-3') on a DNA molecule that is recognized and cut by the restriction enzyme HinF1. When HinF1 cleaves the DNA at its site, it generates specific DNA fragments with defined ends that can be useful for molecular biology applications such as DNA analysis or cloning.


Two different restriction enzymes that recognize the same restriction site known as?

Two different DNA sequences


Explain the Molecular mechanism functions of restriction endonucleases?

Restriction Endonucleases recognize certain sites on the DNA or the sequences. For example EcoR1 that recognizes the restriction site GAATTC on any strand of DNA or RNA.


What is the restriction site of the restriction enzyme Hae III?

The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.


What is the term that describes the sequence of DNA that a restriction enzyme finds and cuts?

Template Sequence


What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?

The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.