S. mitis stains purple and therefore is a gram positive bacteria
Gram stains are typically performed on fresh bacterial cultures because as bacteria age, their cell walls may deteriorate and lose the ability to retain the crystal violet dye used in the Gram staining procedure. This can result in unreliable or inaccurate staining results. Additionally, older cultures may contain cellular debris or contaminants that can interfere with the staining process.
Half Answer: There are both Gram positive and Gram negative stains that are used to identify different types of Bacteria. They depend upon 'membrane content' - some stains highlight only the nuclear envelope.
Water is used in Gram stains primarily to rinse and remove excess dye and other reagents from the bacterial smear. This helps to ensure that the staining process highlights the differences between Gram-positive and Gram-negative bacteria, as the water aids in the clarity of the final results. Additionally, it helps to prevent background staining, allowing for better visualization of the bacterial cells under a microscope.
If you have done your gram stain properly the mycobacterium will appear to be gram positive, however an acid fast stain is necessary because of the extremely long hydrocarbon chains and the high lipid content in the cell wall. The high lipid content makes the cell wall waxy. Both of these make the cell fairly impermeable to stains.
Neither - the influenza VIRUS is not typed by gram stains - only bacteria are.
Crystal violet and safranin are functionally analogous pair of stains in the Gram staining technique. Crystal violet stains gram-positive bacteria purple/blue, while safranin counterstains gram-negative bacteria pink/red.
Its the primary stain of the procedure. IT stains the Gram positive organisms
S. mitis stains purple and therefore is a gram positive bacteria
Gram stains are typically performed on fresh bacterial cultures because as bacteria age, their cell walls may deteriorate and lose the ability to retain the crystal violet dye used in the Gram staining procedure. This can result in unreliable or inaccurate staining results. Additionally, older cultures may contain cellular debris or contaminants that can interfere with the staining process.
Gram staining protozoans yield variable results. Endospore, capsule, and AF stains will yield different results, as these stains are use on bacteria. Malaria is caused by a protozoan.
Gram positive: dark purple Gram negative: light pink You may need to do your gram stain over again. It should be either dark purple or light pink.
Half Answer: There are both Gram positive and Gram negative stains that are used to identify different types of Bacteria. They depend upon 'membrane content' - some stains highlight only the nuclear envelope.
The Gram stain
Pseudomonas aeruginosa does not form endospores. In Gram staining, it typically stains gram-negative due to its thin peptidoglycan layer and outer lipid membrane.
Gram stains are used to analyze bacteria and determine their characteristics. The most popular use of a gram stain is to determine the thickness of a cell wall in bacteria.
By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.