Chromatography

argon

--------------------------------------------

Any of the elements in column 18 of the Periodic Table, plus gasses made form some compounds (eg CO2) and some nonreactive gases such as Nitrogen could be considered as inert.

If the developing chamber is left uncovered during paper chromatography, the solvent will evaporate faster, leading to a higher concentration of the mobile phase at the starting line. This can result in shorter migration distances for the cations, leading to lower Rf values compared to when the chamber is covered and the conditions are more controlled.

Chromatography paper can be purchased from scientific supply companies, specialty chromatography suppliers, or online retailers that sell laboratory supplies. Popular options include sites like Amazon, Sigma-Aldrich, and VWR International.

In column chromatography, the enzymes are made to pass through the column without occurrence of bubbles. These enzymes are obtained at the end of the process by slowly advancing through every column.

Thin-layer chromatography (TLC) and gas chromatography (GC) are commonly used to separate lipids based on their chemical properties such as polarity and volatility. TLC separates lipids based on their partitioning between a stationary phase and a mobile phase, while GC separates lipids based on their boiling points and vapor pressures in a gas phase.

TLC has following advantages over paper chromatography: greater speed greater sensitivity for many substances than paper Small sample requirement Usually sharper preparation Different kind of reagents can be applied without damaging the plate

well first u do the whole chromatography thing. then u divide the solvent(distance the water traveled) by the distance the green tea components traveled alone. Then u should get you answer and should be between 0-1. Have a nice day;)

They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.

Thin-layer chromatography consists of a stationary phase immobilised on a glass or plastic plate and a solvent as mobile phase. The sample, either liquid or dissolved in a volatile solvent, is deposited as a spot on the stationary phase. The constituents of a sample can be identified by simultaneously running standards with the unknown spot. One edge of the plate is then vertically placed in a solvent reservoir and the solvent moves up the plate by capillary action. When the solvent front reaches the other edge of the stationary phase, the plate is removed from the solvent reservoir. The separated spots are then visualized with ultraviolet light or by a suitable reaction procedure....:)

Chromatography is used in pharmaceutical industries for the synthesis of various drugs to check whether the product is synthesized from the reactants or not. It is used in petrochemical industries to check the purity of the fuel and its additive. It is also used by forensic scientists & helps them in solving different cases.

The first chromatography used was with polar stationary phase and non polar mobile phase, called normal phase. So, later when this was reversed by using polar mobile phase and non polar stationary phase was called reversed phase.

Although reversed phase implies that it is less used, it is not the case. RPLC rose to success around the 1970s as NPLC dropped off.

u have to see the ink that comes and see which 2 colours are same and next to each itherr

That is for paper chromatography!

In gas chromatography you determine the concentration of the compound of interest in the sample by determining the area under the peak (AUC). The bigger the peak the higher the concentration. You compare this value to the standard curve that should be run at the same time, to determine the quantitative concentration value. The order of the peaks in the chromatograph tells you which compounds exited first and which where "retained" longer. Retention time is specific for a given compound under the conditions of the GC and can be used to identify your compound of interest.

There are two big advantages of thin later chromatography. They are 1. simplicity and 2. economy. The experimental setup is simle and eay to perform since it only involves spotting the stationary phase with the sample and placing one edge of the stationary phase plate in the mobile phase reservoir. The process is economical since the equipment required for the experiment is rather minimal. It only requires the TLC stationary phase, which is many cases is Silica and can be bought from a variety of commercial analytical chemistry lab suppliers. The mobile phase is generally prepared in the laboratory. In addition, the mobile phase travells up the stationary phase by capillary action, unlike in other chromatography setups where the mobile phase has to be pumped.

Gel filtration or gel permeation is a process by which molecules can be separated according to their size(molecular weight) and sometimes shape. small molecules get trapped and slowed down in the pores of the beads but large molecules simply flow down the column..therefore larger molecules come out first.

Rf value, or retention factor, is a measure used in chromatography to quantify the separation of components in a mixture. It is calculated by measuring the distance a compound travels up the chromatography plate relative to the distance the solvent front travels. Rf value is specific to the solvent system and chromatography conditions used.

Chromatography is used in forensic science. It seperates ink and can remeble links with criminals helping to solve cases. Answer: Chromatography is used in pharmaceutical industries for the synthesis of various drugs to check whether the product is synthesized from the reactants or not. It is used in petrochemical industries to check the purity of the fuel and its additive. It is also used by forensic scientists & helps them in solving different cases.

The Rf value cannot be determined since the distance of the solvent may overlay in the paper.

Gas-liquid chromatography is also called vapor-phase chromatography because it involves the separation of components of a sample based on their volatility in the vapor phase. In this technique, a gas (typically an inert carrier gas) is used to carry the sample through a liquid stationary phase, where separation occurs based on differences in partitioning between the gas and liquid phases. By using a volatile mobile phase, gas-liquid chromatography allows for the analysis of compounds with relatively low boiling points.

Butanol is used as a solvent in paper chromatography because it can dissolve a wide range of compounds. It helps to carry the sample and allow it to migrate up the paper. Butanol also helps in separating the components of the sample by interacting differently with different compounds.

High-performance liquid chromatography (HPLC) works by pumping a liquid sample through a column packed with tiny particles. These particles have different affinities for the components of the sample, causing them to separate as they pass through the column. The separated components are then detected by a detector, which produces a chromatogram. HPLC is commonly used in analytical chemistry to separate and quantify compounds in a mixture.

A series of solvents with an increasing degree of polarity, generally used to explain solvent strength in liquid-solid or adsorption chromatography. A nonpolar solvent such as pentane would be at one end of the scale; dichloromethane would be an intermediate solvent; a strongly polar solvent, such as water, would be at the other end of the scale. Thus, when developing a method or running a gradient, an eluotropic series is useful for selecting solvents.

Chromatography is an analytical tool used to separate and analyze complex mixtures. It works based on the principle that different components in a mixture will move at different rates through a stationary phase when subjected to a mobile phase. By analyzing the resulting separation pattern, chromatography can provide valuable information about the composition and identity of the mixture being analyzed.

The Rf value would not be the same for every solvent as there are factors that allow each solvent to be unique. The attractive force, particle size and solubility of each solvent will create different results each time.

can you explain how the kidneys remove wastes and keep fluids and salts in balance?