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after isolation assumed quantity of milli q is added to it and is dissolved by keeping the eppendorf in a beaker containing lukewarm water,then the concentration of DNA is estimated with the help of picodrop or nanodrop
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How do carbohydrates affect DNA isolation from plant cells?
They are sticky and interfere with the purification process.
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
What is the principle involved in the isolation procedure?
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
Why chilled ethanol is added for DNA isolation?
to precipitate extracted DNA
What is the Role of NaCl in DNA isolation?
NaCl provides Na+ions which form ionic bond with the negatively charged phosphate of DNA,thus neutralizing the effect of negative ,negative repulsion of DNA and helps the DNA molecules to come closer and compact to simplify our process of DNA isolation... BY FARHANA RIYAZ JEZAN UNIVERSITY SAUDI ARABIA.
What are the function of sodium acetate in dna isolation?
Sodium acetate buffer helps by reacting with the membrane protein and precipitating them, thus facilitating the dna isolation.
Role of chloroform in DNA isolation?
It solubilizes lipids and a lot of proteins to remove them from the DNA.
Why heparin can not be used in DNA isolation methods?
heparin may be extrected along with DNA
