0
Eosin Methylene Blue Agar (EMB) was developed by Holt-Harris and Teague.1 This formula contains lactose and sucrose with two indicator dyes, Eosin Y and Methylene Blue. The use of Eosin Y and Methylene Blue as indicators produced sharp and distinct differentiation between colonies of lactose fermenting and nonfermenting organisms. Sucrose is included to detect coliforms that ferment sucrose more readily than lactose. EMB Agar is selective due to the presence of an inhibitor and differential based on the ability of some organisms to ferment carbohydrates with the absorption of an Eosin Y and Methylene Blue complex.
Wiki User
∙ 15y ago
Add your answer:
Earn +20 pts
What function of emb agar?
function of eosin methylene blue agar (EMB)
The agar used most often for throat cultures is the?
Emb agar
What conclusions may be drawn if no growth appeared on MacConkey agar and emb agar after inoculation of the media and an incubation period?
The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.
Which bacteria gives dark puple colour on EMB agar medium?
Escherichia coli-Salmonella-Shigella-Enterobacter
What is the difference between EMB agar and MacConkey agar?
So here is the deal, same use different recipies different results to do the same thing. I'll just post some lecture notes and hope that helps:MacConkey's Agar (enterics)Selective for gram negativeCrystal violet and bile salts prevent non-entericsDifferential for lactose fermentationNon-fermenter --> white/clear coloniesFermenter--> red/pink coloniesEosin Methylene Blue (EMB) (Weakly selective for intestinal pathogens)Selective for gram negativeEosin and methylene blue inhibit gram positive growthDifferential for lactose fermentationLactose fermenters produce a green-metallic sheenNon-fermenters do notNOTE: EMB Agar is not in common use anymore
Does Mycobacterium phlei grow on EMB agar?
yes it can be grow in EMB agar.
What function of emb agar?
function of eosin methylene blue agar (EMB)
The agar used most often for throat cultures is the?
Emb agar
How does yersinia look on nutrient agar EMB agar and MacConkey agar?
Colorless, it doesnt ferment lactose.
Which gram positive bacteria grows on macconkey and EMB?
Gram + bacteria do NOT grow on EMB agar, EMB agar inhibits their growth, and is selective for Gram -
Is EMB agar a defined or undefined medium?
undefined
State the usefulness of EMB agar?
EMB agar or Eosin methylene blue agar is to see which gram negative is lactose fermenters by colonies appearing a purple, black color. It also inhibits the growth of gram positive bacteria.
What media is used to look for fecal contamination of a sample?
EMB Agar
What conclusions may be drawn if no growth appeared on MacConkey agar and emb agar after inoculation of the media and an incubation period?
The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.
What conclusion may be drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period?
The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.
What ingredients of EMB agar inhibit the growth of gram positive organisms?
methylene blue
What is the difference between EMB agar and MacConkey agar?
So here is the deal, same use different recipies different results to do the same thing. I'll just post some lecture notes and hope that helps:MacConkey's Agar (enterics)Selective for gram negativeCrystal violet and bile salts prevent non-entericsDifferential for lactose fermentationNon-fermenter --> white/clear coloniesFermenter--> red/pink coloniesEosin Methylene Blue (EMB) (Weakly selective for intestinal pathogens)Selective for gram negativeEosin and methylene blue inhibit gram positive growthDifferential for lactose fermentationLactose fermenters produce a green-metallic sheenNon-fermenters do notNOTE: EMB Agar is not in common use anymore
