Enzyme turnover is a replacement of new for 'old'.
turnover number
As enzyme concentration increases the more active sites there are avalible, so the rate of reaction increases. therefore the turnover number increases.Hope it helped!TashaThe above it not true. The turn over number is Vmax/Et so if the enzyme concentration is doubled the velocity will also be doubled. Therefore the turn over number will remain constnat.
Adenosine deaminase is an enzyme(EC3.5.4.4) involved in purine metabolism. It is needed for thebreakdown of adenosinefrom food and for the turnover of nucleic acidsin tissues.
Depends on enzyme purity, activity and incubation conditions.
The optimum pH and optimum temperatures of an enzyme are simply levels that the enzyme work most effectively at. These levels contribute to factors such as the enzyme's protein structure. Different levels of pH and temperature can alter the integrity of the protein, this process is called denaturation. When an enzyme undergoes denaturation, its level of activity is inhibited by failure of the substrate to effectively attach itself to the active site.
turnover number
As enzyme concentration increases the more active sites there are avalible, so the rate of reaction increases. therefore the turnover number increases.Hope it helped!TashaThe above it not true. The turn over number is Vmax/Et so if the enzyme concentration is doubled the velocity will also be doubled. Therefore the turn over number will remain constnat.
The turnover number refers to the enzyme activity during a biochemical process. When a protein is denatured it can no longer function and so the turnover number goes down.
You take out a ruler. & you measure it. That is the most sufficent way i have done this.
Adenosine deaminase is an enzyme(EC3.5.4.4) involved in purine metabolism. It is needed for thebreakdown of adenosinefrom food and for the turnover of nucleic acidsin tissues.
Kcat : First-order rate constant (kcat) reflecting the turnover number of the enzyme, or the number of molecules of substrate converted to product per unit time, when the enzyme is working at maximum efficiency, which called also turnover number. Kcat = Vmax / [E]total (Letian) Kcat : First-order rate constant (kcat) reflecting the turnover number of the enzyme, or the number of molecules of substrate converted to product per unit time, when the enzyme is working at maximum efficiency, which called also turnover number. Kcat = Vmax / [E]total (Letian)
Catalytic turnover constant. See the article on enzyme kinetics. http://en.wikipedia.org/wiki/Kcat
Catalase, which can convert about 40 million H202 molecules to H20 & O2 in 1 Sec.
Depends on enzyme purity, activity and incubation conditions.
Assuming that the turnover number is 5000 per second, which you did not specify, than the number of molecules that can be reached by the enzyme in 5 minutes is just this:5 minutes * 60 seconds/minute * 5000 turnover/second = 150,000 turnoversThis is because 1 turnover counts as one molecule reached by the enzyme.If instead the turnover number was 5000 per minute, then it would be:5 minutes * 5000 turnovers/minute = 25,000 turnovers.If it was 5000 per hour, then it would be:5 minutes * 1 hour/60 minutes * 5000 turnovers/hour = 416.7 turnovers.
The optimum pH and optimum temperatures of an enzyme are simply levels that the enzyme work most effectively at. These levels contribute to factors such as the enzyme's protein structure. Different levels of pH and temperature can alter the integrity of the protein, this process is called denaturation. When an enzyme undergoes denaturation, its level of activity is inhibited by failure of the substrate to effectively attach itself to the active site.
What is turnover intention?