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peptone iron agar is used to demonstrate the production of hydrogen sulfide. ıt is more sensitive than lead acetate agar. some organisms have the enzyme to liberate hydrogen sulfide from sulfur containing organic compounds-such as protein-. when H2S is liberated by the enzyme,, the sulfur of the H2S combines with the iron in the PIA medium to form an iron sulfide. The iron sulfide is manifested as a black precipitate in the medium. so you can use in the separation and identification of bacteria. Peptone Iron Agar Approximate Formula* Per Liter Peptone ................................................................... 15.0 g Proteose Peptone ....................................................... 5.0 g Ferric Ammonium Citrate ........................................... 0.5 g Sodium Glycerophosphate ......................................... 1.0 g Sodium Thiosulfate .................................................... 0.08 g Agar ......................................................................... 15.0 g
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Why would peptone ion agar be used in a biochemical test?
peptone agar is used to show the production of hydrogen sulfide
What are selective media for aspergillus?
Peptone glucose agar (PGA) medium
What is the nutrients in nurtient agar?
Beef extract for carbohydrates Peptone for protein and NaCl as salt
What is the composition of modified nutrient glucose agar media?
Modified nutrient glucose agar is composed of agar, water, glucose, extracts, peptone and sodium chloride. Modified nutrient glucose agar allows organisms to grow.
What is the indicator that turns the agar medium pink with copper?
MacConkey agar contains bile salts, lactose, peptone. crystal violet dye, and neutral red dye. The agar appears a medium pink with a copper undertone.
Why did you stab the peptone iron agar instead of inoculating only the surface?
Because the peptone iron agar is used to detect ANAEROBIC bacteria. If you stab it deep into the agar you allow the bacteria to grow in the absence of oxygen. If you only inoculated the surface the bacteria wouldn't grow.
Why would peptone ion agar be used in a biochemical test?
peptone agar is used to show the production of hydrogen sulfide
What is the source of nutrients in nutrient agar?
peptone, yeast extract,meat extract..
The source of nutrients in nutrient agar is?
Peptone and beef extract
Role of peptone in nutrient agar?
Peptone is the simplified source of protein and can be readily uptake by the microorganism. In nurtient agar it is the basic component/nutrient after which beef or yeast extract is used which are supplementary nutrients.
What are selective media for aspergillus?
Peptone glucose agar (PGA) medium
What is the chemical composition of malt extract agar?
thats a good question but unforunately i dont now the answer
What is the nutrients in nurtient agar?
Beef extract for carbohydrates Peptone for protein and NaCl as salt
Ingredients of sabouraud's agar?
sabourauds agar contains the following : peptone : 1 g glucose : 4 g agar :3 g d/w : 100ml PH : 5.4
What is the composition of modified nutrient glucose agar media?
Modified nutrient glucose agar is composed of agar, water, glucose, extracts, peptone and sodium chloride. Modified nutrient glucose agar allows organisms to grow.
What is the indicator that turns the agar medium pink with copper?
MacConkey agar contains bile salts, lactose, peptone. crystal violet dye, and neutral red dye. The agar appears a medium pink with a copper undertone.
What is the nitrogen source in PEA agar?
Pancreatic digest of casein, papaic digest of soybean meal, beef extract, peptone
