The gel electrophoresis technique is developed by Joe Sambrook and Bill Sugden. They used agarose gel electrophoresis to separate DNA fragements. The most easily way the macromolecules through the gel porous starts with smallar molecules than larger molecules.
Precise information about invention of gel electrophoresis is not known. However, the first documented use of the profess was in the early 1930's (with sucrose).
In 1959, Milan Bier composed a history of the process and cites its origin in the 1800's.
The technique was invented by Grant Henry Lathe and Colin R Ruthven, working at Queen Charlotte's Hospital, London.[3][4] They later received the John Scott Award for this invention.[5] While Lathe and Ruthven used starch gels as the matrix, Jerker Porath and Per Flodin later introduced dextran gels;[6] other gels with size fractionation properties include agarose and polyacrylamide. A short review of these developments has appeared.[7]
There were also attempts to fractionate synthetic high polymers; however, it was not until 1964, when J. C. Moore of the Dow Chemical Company published his work on the preparation of Gel Permeation Chromatography (GPC) columns based on cross-linked polystyrene with controlled pore size,[8] that a rapid increase of research activity in this field began. It was recognized almost immediately that with proper calibration, GPC was capable to provide molar mass and molar mass distribution information for synthetic polymers. Because the latter information was difficult to obtain by other methods, GPC came rapidly into extensive use.[9]
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Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
it is a separation technique
electrophoresis
electrophoresis=)
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Gel Electrophoresis
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
Electrophoresis - journal - was created in 1980.
B. J. Haywood has written: 'Electrophoresis - technical applications' -- subject(s): Abstracts, Bibliography, Electrophoresis 'Electrophoresis-technical application' -- subject(s): Bibliography, Electrophoresis
it is called " electrophoresis"
yes for example 2D gel electrophoresis
Gel electrophoresis
Paper electrophoresis is used to analyze scientific experiments. One use in scientific experiments for paper electrophoresis is to determine the presence of HIV from blood samples.
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.