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Why was it important to find an enzyme that would cut the plasmid at only one site?
Wiki User
∙ 13y ago
Wiki User
∙ 13y ago
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In a paper on catalytic activity of an enzyme in which section would you find detailed information on how the enzyme was prepared and handled in the experiment?
Material and method
Why you are using enzyme blank and substrate blank instead of using a single blank to find out the enzyme activity?
I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?
Why would it be important for a commercial fisherman to have a dichotomous key on the boat with them?
It would be important because he can identify various types of fish and can recognize if some of the fishes are poisonous.
What enzyme adds base pairs in order to create the new strands in DNA replication?
DNA polymerase is the enzyme that replicates the pattern of the nucleotide bases. Its function is find the correct base, and then to bond it onto the original strand.
What would you find in a cell from a leaf that you would not find in your fingernail?
You would find a cell wall and chloroplasts.
The restriction enzyme used in constructing hybrid molecules of certain gene sequences and plasmid DNA acts by?
opening DNA molecules at specific sites,leaving sticky ends exposed. find me on kik one1992
In a paper on the catalytic activity of an enzyme, in which section would you find detailed information on how the enzyme was prepared and handled in the experiment?
materials and methods
In a paper on catalytic activity of an enzyme in which section would you find detailed information on how the enzyme was prepared and handled in the experiment?
Material and method
How do you find suitable plasmid?
if you are looking for a repository grade plasmid you should search the link below but beware of what you buy through some of the more shady vendors here: importer.ec21.com/buy_leads/plasmid.html
Can genetic engineering techniques treat cystic fibrosis?
Yes, there are two similar techniques in which i am aware of.AdenovirusesFirstly adenoviruses are made harmless by interefering with a gene involved in replication. A healthy from of the CFTR gene is extracted and cut with restriction endonucleases, the same enzyme is used to cut a bacterial plasmid. The gene and plasmid are mixed together along with DNA ligase to anneal the phosphosugar framework of the DNA fragment and bacterial plasmid. The plasmid is then mixed with epithelial cells. The plasmid is then isolated and purified and places into adenoviruses. These are then placed onto the nostrils of individuals with cystic fibrosis. The viruses find their way to epithelial cells in the airways and injected their DNA. The DNA contains the functional CFTR gene, the cells can then produce fucntional CFTR proteins.LiposomesA healthy gene is extracted from a human. This gene is then inserted into a bacterial plasmid, in a similar manner as discussed above. The bacterial plasmids are then inserted into bacteria. These are allowed to grow and divide, producing large quantities of the plasmid, with the required gene. These plasmids are then extracted and coated in a lipid soluble substance. They are then put into nasal sprays and sprayed onto the nostrils of effected individuals.
How can you obtain large amounts of enzymes if the source is not sufficient for producing them?
You would need to find more sources for the enzyme.
Who are the important people you would find in the mosque?
you would find Muslim people.
Does pjhgeph-c4 have a poly-histidine tag?
where is it from? someone would have to add a his tag on purpose. There are imuno-assays available to detect a his tag. Otherwise find out if the his tag was introduced when it was cloned to a plasmid. You should have access to these papers.
How do you find the enzyme inhibition constant?
Ki = [I]/alpha - 1
Where are you likely to find the enzyme cytochrome oxidases?
Cytochrome Oxidase are most likely to be found in mitochondria and important in cell respiration as an agent of electron transfer from certain cytochrome molecules to oxygen molecules
How do you get the telekinesis plasmid in BioShock?
There is no teleport plasmid in any of the Bioshock games. In the Fontaine Futuristics level in Bioshock 2, there is an unstable teleport plasmid sitting on a desk. If you interact with it, it will teleport somewhere in the room. Keep following it and it will lead you to the vending expert 2 gene tonic.
Why would it be important to find water on the moon?
Life in space dududuudiedjx
