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in DNA estimation by diphenylamine method, standard calf thymus DNA is digested by perchloric acid, but unkown is not digested by perchloric acid, rather rest protocol of diluting with water and treating with diphenylamine and checking aborbance reamins same can anybody say the reason?
Wiki User
∙ 10y ago
Wiki User
∙ 13y agowhen DNA is treated with DPA under acidic conditions, a blue colour complex is formed. this colour complex shows absorption maxima at 595nm. only 2'- deoxyribose of purenes will give this reaction hence the value obtained is half of the total 2'- deoxyribose present.
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What is the reaction mechanism of orcinol in the estimation of RNA?
Furfural cannot be formed from ribosides until the glycosidic linkage has been split; the purine-ribose links of RNA are easily hydrolysed by hot acid, while the pyrimidine-ribose links are much more resistant, and the orcinol method is commonly supposed to determine only the purine-bound ribose of RNA.
What is a method of making any copies of DNA?
Polymerase chain reaction (PCR) is the method of making copies of DNA.
Dideoxyribonucleotide chain-termination is a method of?
Sequencing DNA (Sanger Sequencing Method).
What is the most commonly used method of cloning?
recombinant dna technology
Which is a method of preparing DNA for forensic analysis?
polymerase chain reaction
Why do you use dpa for estimation of DNA other than oricinol?
Only the deoxyribose after heating becomes furfural which then reacts with DPA and produces dark blue coloured compond which can be estimated on the other hand one can say that orcinol does't reacts with deoxyribose similarly DPA does't reacts with ribose
What is front end estimation?
Front end estimation- An estimation method in which the front digits are added or subtracted
What are the methods for estimation of DNA?
diphenylamine reagent
Is lowry's protein estimation an invasive method?
No. It is a non-invasive method
What is a method for math in a estimation?
round or rounding
What is an invasive method of protein estimation?
gand mara
Hb estimation by drabkin's method?
Procedure to estimate haemoglobin in blood by cyanomethaemoglobin method
Can one cost estimation method can be used in combination with another method?
Yes. True
One cost estimation method can be used in combination with another method.?
Yes. True
Can one cost estimation method be used in combination with another method?
Yes. True
What are the methods of estimation of reducing sugar?
by comparing the colours or the amount of precipitate
What is the method of estimation using a representative section of a whole?
sampling
