Function of iodine in gram staining?

Gram's Iodine acts as a mordant in gram staining. Read More

Iodine serves as a mordant by binding to the crystal violet. An insoluble iodine-crystal violet complex is formed that allows gram positive bacteria to remain purple after this step in the gram staining procedure. Read More

The acetone is used as the decoloring part of gram staining. Either the acetone will wash away the previous step of iodine and crystal violet or it will not wash off the crystals. Read More

Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out. Read More

Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex. Read More

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In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet . Read More

fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process. Read More

it acts as amordant for gram positive bacteria by inhancing the staining of crystal violate Read More

Iodine solution is used as mordant in grams staining. At first smear is stained with crystal violet and then with iodine. When gram positive bacteria are treated with ethanol, the alcohol is thought to shrink the pores of thick peptidoglycan. Thus the dye-iodine complex is retained during this short decolorization step and the bacteria remain purple. In contrast, the gram negative get decolourized and when counterstain with saffranine it shows pink colour. So iodine from… Read More

what is the function of ethyl alcohol Read More

Gram positive bacteria after gram staining will appear purple in color. It appears purple because of the iodine and crystal violet dyes used in earlier steps of the gram staining procedure. Read More

to fix the bacteria Read More

identification of bacteria strains Read More

Acetone is used for decolouring method, washing away the iodine-crystal violet complex formed in gram negative bacteria. so that the gram negative bacteria can be then stained with safranin or fuchsin. Read More

I believe that Iodine helps the cell take up the Crystal Violet. If Iodine is not used and you did everything else the right way that you would get a false gram negative. The Violet would not get taken up like it should and the Safranin would end up staining a Gram positive cell red. Read More

Crystal violet, Gram's iodine, alcohol, and safranin Read More

It's one of the differentiating dyes that shows a bacterium is gram positive by staying purple at the end of the staining process. Read More

The primary stain is crystal violet + iodine. The secondary is safranin. Read More

It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple… Read More

It is a colouring reagent. Methylene blue is useful for the staining of bacterial cells. it is one of the staining reagent of Gram staining technique. Read More

Thomas Francis McNamara has written: 'Iodine and the quantitative gram reaction' -- subject(s): Iodine, Stains and staining (Microscopy) Read More

Safranin stains G- bacteria red. Read More

Gram staining is important because to differentiate between gram positive and gram negative Read More

Gram staining is done to determine Read More

The steps in Gram staining are: 1. crystal violet added to the smear 2. iodine, the mordant (this fixes the violet) 3. a decolorizer made of acetone and alcohol 4. safranin, the counterstain If the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin. Bacteria are grouped in 4 groups by… Read More

it will interact with the lipid of the membrane. gram negative will lose its outer membrane n easily counterstained while gram positive will not Read More

Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938) Read More

Normally gram positive bacterium have a thick peptidoglycon layer where as gram negative bacterium has a thin peptidoglycon layer . The lipopolysaccharide content is more in gram negative bacteria but its not present in gram positive . There is a staining procedure called gram staining which differentiates between a gram positive from gram negative bacteria . In this staining procedure the bacteria is treated with crystal violet , iodine , ethanol and safranin in which… Read More

Gram staining is a process for the detection of structure and morphology of bacteria. Read More

Usually iodine compounds leave a yellow-brown stain on whatever they touch. Non staining will reduce that chance. Read More

If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells. Read More

list the steps of the gram staining procedure Read More

Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin Read More

Gram stain is used to determine whether the cell is gram positive or negative. Gram positive is when the cell has very thick layers of peptidoglycan, and gram negative when the layers are thin. In the staining process, those that decolorize easily are gram negative, and the one that retain the primary stain are gram positive. The acid fast staining is a techique that stains only the gram positive and only the genuses of mycobacterium… Read More

It removes the crystal violet (primary stain) from gram negative bacteria. It does not remove Crystal violet as easily from gram positive bacteria, because the highly peptidoglycan walls of gram positive bacteria interact with crystal violet and iodine to form a strong bond (CV-Iodine complex). So, gram positive cells hold on to the stain instead of letting it wash away. Read More

A basic dye used in gram staining is crystal violet. Read More

Gram staining is the way for people to get knowledge about their specimen , if it is gram negative or gram positive. Gram positive have thick walls and stains purple, gram negative has thin walls and will turn pink after gram staining and bacteria with spores and endospores will show up clear or see through because it cant stain with gram staining dye. Read More

Cultures that are older have the possibility to turn from Gram Postive to Gram Negative thus altering the results of the Gram staining test. Read More

No. It is a staining on the cell itself. Read More

In all bacteria that are Gram stained all the steps are used. It isn't until the end that you will know if they are Gram+ or Gram-. In Gram staining, all bacterial cells take up the crystal violet. Iodine is then added; it acts as a mordant, a chemical that helps retain the stain in certain cells. Those structures that cannot retain crystal violet are decolorized with 95% ethanol or an ethanol- acetone solution, rinsed… Read More

The mordant in the negative staining procedure is Gram's iodine. Read More

Iodine stains starch contained in cells. Iodine is also used to distinguish between Gram negative and Gram positive bacteria. The Gram stain contains iodine. Read More

$.0016 not even a cent for a gram of iodine! Read More

The iodine in the gram stain serves as a mordant or fixative. Read More

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining Read More

No. Iodine acts as the mordant, or the agent used to drive the stain into the bacterial cells. If you use the iodine first, it will take up all the space inside the cells, therefore rendering the stain useless. The stain would have nowhere to go. Read More

The nucleus and cell wall of elodea cell can be seen more clearly after staining it with iodine. Read More

To determine if bacteria is gram positive or gram negative Read More

Gram negative bacteria stain red or pink because of the retaining counter staining dye (Safranin). Read More